Increased oxygen tension attenuates acute ultraviolet-B-induced skin angiogenesis and wrinkle formation
Increased oxygen tension attenuates acute ultraviolet-B-induced skin angiogenesis and wrinkle formation
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DOI：
10.1152/ajpregu.00199.2010
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发表时间：
2010-08-01
影响因子：
2.8
通讯作者：
Ishii, Naokata
中科院分区：
文献类型：
--
作者：
Kawada, Shigeo;Ohtani, Masaru;Ishii, Naokata
急性紫外线(UV)-B辐射会导致皮肤皱纹的形成，并伴随皮肤血管的增生。这项研究报告说，增加真皮氧分压减弱了急性UVB诱导的血管生成和皱纹的形成。24只无毛小鼠(HOS：HR-1)随机分为3组：1)对照组，2)中波紫外线照射(UVB)组，3)中波紫外线照射+高氧暴露(UVB+HO)组。小鼠背部接受UVB照射，每周照射3次，持续5wk。为了提高皮肤氧分压，每次UVB照射后，立即将小鼠暴露于高氧(90%氧气)中2小时。与对照组相比，高氧暴露使皮肤氧分压增加了约10倍。经过5周的UVB照射后，皱纹形成程度和表皮厚度显著增加，而高氧暴露减轻了这些增加。组织中三磷酸腺苷浓度和血管生成仅UVB组较对照组明显升高。与对照组相比，UVB组和UVB+HO组血管生成关键分子低氧诱导因子-1αmRNA的表达显著增加，但仅UVB组显著升高。与对照组相比，UVB组和UVB+HO组血管生成的关键分子基质金属蛋白酶-2和-9的活性没有增加。各组的活性I型胶原酶活性和可溶性胶原含量大致相似。这些结果表明，增加真皮氧分压可减少急性UVB照射后的血管生成和皱纹的形成。
Acute ultraviolet (UV)-B irradiation causes skin wrinkle formation associated with hyperplasia of cutaneous blood vessels. This study reports that increased dermal oxygen tension attenuates acute UVB-induced angiogenesis and wrinkle formation. Twenty-four hairless mice (HOS:HR-1) were assigned to 3 groups: 1) control group, 2) UVB-irradiated (UVB) group, and 3) UVB-irradiated and hyperoxia-exposed (UVB+HO) group. The backs of the mice were exposed to UVB irradiation 3 times per week for a 5-wk period. To increase dermal oxygen tension, the mice were exposed to hyperoxia (90% oxygen) for 2 h immediately after each UVB irradiation. Hyperoxic exposure increased dermal oxygen tension by about 10 times compared with the control level. Degree of wrinkle formation and epidermal thickness increased significantly after a 5-wk UVB-irradiation period, whereas hyperoxic exposure attenuated these increases. Tissue adenosine triphosphate concentration and angiogenesis increased significantly only in the UVB group compared with the control group. Although the expression of hypoxia inducible factor-1 alpha mRNA, a key molecule for angiogenesis, increased significantly in the UVB and UVB+HO groups compared with the control group, the protein level increased significantly only in the UVB group. The activity of matrix metalloproteinase-2 and -9, critical molecules for angiogenesis, did not increase in the UVB and UVB+HO groups compared with the control group. Active type 1 collagenase activity and soluble collagen content in all of the groups were roughly similar. These results suggest that increased dermal oxygen tension attenuates angiogenesis and wrinkle formation following acute UVB irradiation.