Simple sequence repeats (SSRs) in faba bean: new loci from Orobanche-resistant cultivar 'Giza 402'
Simple sequence repeats (SSRs) in faba bean: new loci from Orobanche-resistant cultivar 'Giza 402'
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DOI：
10.1111/j.1439-0523.2008.01584.x
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发表时间：
2009-04-01
期刊：
影响因子：
2
通讯作者：
Kresovich, S.
中科院分区：
文献类型：
--
作者：
Zeid, M.;Mitchell, S.;Kresovich, S.
对来自钝齿列当抗性品种“吉萨402”的近交系的基因组DNA进行限制性内切酶分析，并富集了含AG的简单重复序列（SSR）。SSR的富集率为54.6%。在“吉萨402”中成功扩增的73个SSR标记中，有54个在10个埃及和西班牙蚕豆基因型中具有多态性，这些基因型广泛应用于齿列当属抗性/感病研究和连锁图谱的构建。多态性信息含量在0.16 ~ 0.72之间，根据这些位点的算术平均数进行的非加权配对聚类分析将亲本F402后代的所有抗性基因型归为一类。在5个欧洲蚕豆基因型中也观察到28个引物对的成功扩增。这里描述的SSR位点将是非常有用的研究，重点是对列当的抗性，遗传多样性的研究，并将提供额外的共显性位点，以补充现有的蚕豆连锁图谱的基础上，主要是显性标记。
Genomic DNA from an inbred line from the Orobanche crenata resistant cultivar 'Giza 402' was restricted and enriched for AG-containing simple sequence repeats (SSRs). The overall SSR enrichment rate was 54.6%. Of 73 selected SSR markers that successfully amplified in 'Giza 402' 54 were polymorphic among a group of 10 Egyptian and Spanish faba bean genotypes extensively used in Orobanche crenata resistance/susceptibility studies and linkage map development. The polymorphism information content ranged from 0.16 to 0.72 and Unweighted Pair-Group Method using Arithmetic averages cluster analysis based on these loci placed all resistant genotypes descended from the parental line F 402 in the same group. Successful amplification from 28 primer pairs was also observed in five European faba bean genotypes. The SSR loci described here will be extremely useful in studies focusing on Orobanche crenata resistance, genetic diversity studies and will provide additional co-dominant loci to complement existing faba bean linkage maps based primarily on dominant markers.