Hyaluronan dynamics during retinal development
Hyaluronan dynamics during retinal development
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DOI：
10.1016/j.brainres.2008.12.023
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发表时间：
2009-02-23
期刊：
影响因子：
2.9
通讯作者：
Zako, Masahiro
中科院分区：
文献类型：
--
作者：
Inoue, Yoko;Yoneda, Masahiko;Zako, Masahiro
我们已经证明了透明质酸结合分子（包括多功能蛋白聚糖、SPACR 和 SPACCAN）在视网膜发育过程中的表达调节。在这里，我们分析了这些时期透明质酸的概况。分别通过狭缝印迹和实时PCR评估不同年龄鸡视网膜中透明质酸和透明质酸合酶（HAS）的表达。使用 Superose-6 柱测定透明质酸的分子大小，并使用组织化学来定位透明质酸的表达。将透明质酸添加到视网膜提取物中后测量透明质酸降解活性。在胚胎视网膜中，透明质酸主要存在于内丛状层和感光层中。内丛状层的表达随着发育和衰老而减少，而感光层的表达保持恒定。从胚胎第 12 天 (E12) 到出生后第 1 天 (P1)，透明质酸的分子大小随着视网膜透明质酸降解活性的增加而减小。在成人视网膜中，透明质酸仅存在于感光层，且其分子尺寸最大。 HAS-2和HAS-3表达从E15到P1增加，但在成年期均减少。因此，在先前证实了多功能蛋白聚糖、SPACR和SPACRCAN的情况下，可以特异性地检测到透明质酸，并且透明质酸的合成和降解受到调节。透明质酸与多功能蛋白聚糖、SPACR 和 SPARCCAN 在视网膜发育过程中密切相关，并且可能对视网膜生理学很重要。 (C) 2008 Elsevier B.V. 保留所有权利。
We have already shown the regulated expressions of hyaluronan binding molecules including versican, SPACR, and SPACRCAN, during retinal development. Here we analyzed the profiles of hyaluronan during these periods. Hyaluronan and hyaluronan synthase (HAS) expressions in chicken retinas at different ages were assessed by slot blot and real-time PCR, respectively. The molecular size of hyaluronan was determined using a Superose-6 column, and histochemistry was used to localize hyaluronan expression. Hyaluronan degradation activity was measured after adding hyaluronan to retinal extracts. In the embryonic retina, hyaluronan was mainly present in the inner plexiform and photoreceptor layers. Expression in the inner plexiform layer decreased with development and aging while expression in the photoreceptor layer remained constant. The molecular size of hyaluronan decreased from embryonic day 12 (E12) to postnatal day 1 (P1) with increasing retinal hyaluronan degradation activity. In adult retinas, hyaluronan was only present in the photoreceptor layer, and its molecular size was the highest. HAS-2 and HAS-3 expressions increased from E15 to P1, but both were reduced in adulthood. Thus hyaluronan was specifically detected where versican, SPACR, and SPACRCAN were previously demonstrated, and the synthesis and degradation of hyaluronan are regulated. Hyaluronan together with versican, SPACR, and SPACRCAN are closely related during retinal development, and may be important for retinal physiology. (C) 2008 Elsevier B.V. All rights reserved.