Poliovirus surveillance by examining sewage specimens. Quantitative recovery of virus after introduction into sewerage at remote upstream location
Poliovirus surveillance by examining sewage specimens. Quantitative recovery of virus after introduction into sewerage at remote upstream location
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DOI：
10.1017/s0950268801005787
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发表时间：
2001-08-01
影响因子：
4.2
通讯作者：
Kangas, A
中科院分区：
文献类型：
--
作者：
Hovi, T;Stenvik, M;Kangas, A
为了评估环境脊髓灰质炎病毒监测的可行性，将已知数量的1型脊髓灰质炎病毒Sabin株冲入赫尔辛基的污水管网。在所有三个单独的实验中，在偏远的下游位置采集并浓缩约100倍的抓取标本，连续四天显示出传染性脊髓灰质炎病毒。至于浓度，一个简单的两相分离方法被认为是至少作为一个几倍的资源需求的聚乙二醇（PEG）沉淀法一样有用。导入病毒的回收率非常高（超过10%）。如果每10000名居民中就有1人排出病毒，那么使用目前的系统，通过检查一个400毫升的污水样本，就有可能在700000人的人口中发现脊髓灰质炎病毒的传播。它的结论是，环境监测是一种敏感的方法来监测沉默的脊髓灰质炎病毒在人口中的流通服务的污水网络。
In order to assess the feasibility of environmental poliovirus surveillance, known amounts of poliovirus type 1, strain Sabin, were flushed into the sewage network of Helsinki. Grab specimens collected at a remote downstream location and concentrated about a 100-fold revealed infectious poliovirus on four successive days in all three separate experiments. As for concentration, a simple two-phase separation method was found to be at least as useful as a several-fold more resource-demanding polyethylene glycol (PEG) precipitation method. Recovery of the introduced virus was remarkably high (more than 10 %). Using the current system, it might be possible to detect poliovirus circulation in a population of 700 000 people by examining a single 400 ml sewage specimen, if I out of 10000 inhabitants were excreting the virus. It is concluded that environmental surveillance is a sensitive approach to monitor silent poliovirus circulation in populations served by a sewage network.