Genetic labeling of Tas1r1 and Tas2r131 taste receptor cells in mice.
Genetic labeling of Tas1r1 and Tas2r131 taste receptor cells in mice.
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DOI：
10.1093/chemse/bjs082
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发表时间：
2012-11
期刊：
影响因子：
3.5
通讯作者：
A. Voigt;Sandra Hübner;Kristina Lossow;I. Hermans-Borgmeyer;U. Boehm;W. Meyerhof
中科院分区：
文献类型：
--
作者：
A. Voigt;Sandra Hübner;Kristina Lossow;I. Hermans-Borgmeyer;U. Boehm;W. Meyerhof
外周味觉系统的表征依赖于不同味蕾细胞类型的识别和可视化。到目前为止，标记味觉受体细胞的遗传策略仅限于甜、酸、咸检测细胞。为了可视化 Tas1r1 鲜味和 Tas2r131 苦味传感细胞，我们生成了动物，其中 Tas1r1 和 Tas2r131 开放阅读框分别被含有荧光蛋白 mCherry 或 hrGFP 的表达盒替换。这些动物使我们能够可视化和量化整个口腔 Tas1r1 和 Tas2r131 细胞群。 Tas1r1-mCherry 细胞主要在菌状乳头中检测到，而 Tas2r131-hrGFP 细胞的数量要多约 4 倍，主要存在于叶状乳头和腭状乳头中。在上颚中，两种细胞类型的分布相似。携带两种重组等位基因的小鼠表现出完全分离的 Tas1r1 和 Tas2r131 细胞群。整个苦味细胞群中只有约 50% 表达 hrGFP，表明苦味受体细胞表达苦味受体库的一个子集。在味觉外组织中，在睾丸中观察到 mCherry 荧光，在睾丸、胸腺、犁鼻器和呼吸道上皮中观察到 hrGFP 荧光，表明只有少数口外部位表达与味觉组织相当的 Tas2r131 和 Tas1r1 受体水平。
Characterization of the peripheral taste system relies on the identification and visualization of the different taste bud cell types. So far, genetic strategies to label taste receptor cells are limited to sweet, sour, and salty detecting cells. To visualize Tas1r1 umami and Tas2r131 bitter sensing cells, we generated animals in which the Tas1r1 and Tas2r131 open reading frames are replaced by expression cassettes containing the fluorescent proteins mCherry or hrGFP, respectively. These animals enabled us to visualize and quantify the entire oral Tas1r1 and Tas2r131 cell populations. Tas1r1-mCherry cells were predominantly detected in fungiform papillae, whereas Tas2r131-hrGFP cells, which are ~4-fold more abundant, were mainly present in foliate and vallate papillae. In the palate, both cell types were similarly distributed. Mice carrying both recombinant alleles demonstrated completely segregated Tas1r1 and Tas2r131 cell populations. Only ~50% of the entire bitter cell population expressed hrGFP, indicating that bitter taste receptor cells express a subset of the bitter receptor repertoire. In extragustatory tissues, mCherry fluorescence was observed in testis and hrGFP fluorescence in testis, thymus, vomeronasal organ, and respiratory epithelium, suggesting that only few extraoral sites express Tas2r131 and Tas1r1 receptors at levels comparable to taste tissue.