An aerotaxis transducer gene from Pseudomonas putida.
An aerotaxis transducer gene from Pseudomonas putida.
复制标题
来自恶臭假单胞菌的趋气传感器基因。
DOI：
10.1111/j.1574-6968.2000.tb08893.x
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发表时间：
2000
影响因子：
2.1
通讯作者：
Harwood,CS
中科院分区：
文献类型：
--
作者：
Nichols,NN;Harwood,CS
从恶臭假单胞菌PRS2000中克隆了嗜气性基因Aer。美联社恶臭变种在毛细管分析中表现出改变的趋气性反应。野性的P。恶臭聚集在气/液界面。相反，空气突变体并没有聚集在界面上，而是在离半月面一段距离的地方形成了一条扩散带。野生型提供了内含子，补充了航空突变体对比野生型更强的有氧反应的反应。塞普。腐烂的Aer序列在整个长度上与大肠杆菌的趋气性(能量趋性)信号转导蛋白Aer相似。氨基末端类似于氧化还原敏感调节蛋白，而羧基末端含有趋化转导分子中存在的高度保守的结构域。
An aerotaxis gene,aer, was cloned fromPseudomonas putidaPRS2000. AP. putida aermutant displayed an altered aerotactic response in a capillary assay. Wild-typeP. putidaclustered at the air/liquid interface. In contrast, theaermutant did not cluster at the interface, but instead formed a diffuse band at a distance from the meniscus. Wild-typeaer, provided intrans, complemented theaermutant to an aerotactic response that was stronger than wild-type. TheP. putidaAer sequence is similar over its entire length to the aerotaxis (energy taxis) signal transducer protein, Aer, ofEscherichia coli. The amino-terminus is similar to redox-sensing regulatory proteins, and the carboxy-terminus contains the highly conserved domain present in chemotactic transducers.