Mel1b and Mel1c melatonin receptors mediate green light-induced secretion of growth hormone in chick adenohypophysis cells via the AC/PKA and ERK1/2 signalling pathways
Mel1b and Mel1c melatonin receptors mediate green light-induced secretion of growth hormone in chick adenohypophysis cells via the AC/PKA and ERK1/2 signalling pathways
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Mel1b 和 Mel1c 褪黑素受体通过 AC/PKA 和 ERK1/2 信号通路介导绿光诱导的鸡腺垂体细胞生长激素分泌
DOI：
10.1016/j.jphotobiol.2021.112322
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发表时间：
2021-10-28
影响因子：
5.4
通讯作者：
Cao, Jing
中科院分区：
文献类型：
--
作者：
Liu, Xinfeng;Wang, Lu;Cao, Jing
先前的研究表明，在单色绿光下，褪黑素（MEL）膜受体1b （Mel1b）和Mel1c促进了鸡腺垂体细胞生长激素（GH）的分泌。然而，这两种受体的细胞内信号通路尚不清楚。因此，我们在体外用MEL、mel1b和mel1c特异性阻滞剂、蛋白激酶A （PKA）抑制剂和腺苷酸环化酶（AC）或AC激活剂处理暴露于单色绿光下的鸡腺垂体细胞，探讨促进GH分泌的信号转导机制。结果表明Mel1b和Mel1c参与了mel介导的绿光诱导鸡腺垂体细胞GH的分泌。然而，MEL增加了环腺苷单磷酸（cAMP）水平，p-PKA蛋白水平被Mel1b特异性拮抗剂阻断，而mel1c特异性拮抗剂不阻断，这表明Mel1b通过AC/cAMP/PKA信号通路影响GH的分泌。此外，Mel1b和Mel1c均激活ERK1/2调节GH的分泌。此外，细胞内和细胞外Ca2+通道也参与了鸡腺垂体细胞GH的分泌。这些结果表明，MEL介导的绿光通过Mel1b/AC/PKA/ERK1/2、Mel1c/ERK1/2以及细胞内和细胞外Ca2+通道信号通路诱导鸡腺垂体GH的分泌。
A previous study showed that melatonin (MEL) membrane receptors 1b (Mel1b) and Mel1c promoted the secretion of growth hormone (GH) in chick adenohypophysis cells under monochromatic green light. However, the intracellular signalling pathways of these two receptors are unclear. Therefore, cultured adenohypophysis cells derived from chickens exposed to monochromatic green light were treated with MEL, Mel1b-and Mel1c-specific blockers, protein kinase A (PKA) inhibitors and adenylate cyclase (AC), or AC activator in vitro to explore the signal transduction mechanism that promote the secretion of GH. The results showed that Mel1b and Mel1c participate in MEL-mediated green light-induced secretion of GH in chick adenohypophysis cells. However, MEL increased cyclic adenosine monophosphate (cAMP) levels, and p-PKA protein levels were blocked by a Mel1b-specific antagonist but not a Mel1c-specific antagonist, which indicated that Mel1b affected the secretion of GH via the AC/cAMP/PKA signalling pathway. Moreover, Mel1b and Mel1c both activated ERK1/2 to regulate the secretion of GH. In addition, intracellular and extracellular Ca2+ channels were also involved in secretion of GH in chick adenohypophysis cells. These results demonstrate that the MEL mediated green light-induced secretion of GH in chick adenohypophysis via the Mel1b/AC/PKA/ERK1/2, Mel1c/ERK1/2, and intracellular and extracellular Ca2+ channel signalling pathways.