Restriction Maps of Five Autographa californica MNPV Variants, Trichoplusia ni MNPV, and Galleria mellonella MNPV DNAs with Endonucleases SmaI, KpnI, BamHI, SacI, XhoI, and EcoRI
Restriction Maps of Five Autographa californica MNPV Variants, Trichoplusia ni MNPV, and Galleria mellonella MNPV DNAs with Endonucleases SmaI, KpnI, BamHI, SacI, XhoI, and EcoRI
复制标题
具有核酸内切酶 SmaI、KpnI、BamHI、SacI、XhoI 和 EcoRI 的五种苜蓿夜蛾 MNPV 变种、粉纹夜蛾 MNPV 和大蜡螟 MNPV DNA 的限制性图谱
作者：
Gale E. Smith;M. Summers
苜蓿银纹夜蛾核型多角体病毒 (AcMNPV) E2 DNA 的限制性位点被绘制为内切核酸酶 SmaI、KpnI、BamHI、SacI、XhoI 和 EcoRI。确定了其他四种 AcMNPV 变体、粉纹夜蛾 (TnMNPV) 和大蜡螟 (GmMNPV) 基因组的限制性图谱，并将其与 AcMNPV E2 DNA 的核酸内切酶切割图谱进行了比较。通过聚丙烯酰胺凝胶电泳分析，AcMNPV变种S3、E2、S1、M3和R9的病毒结构多肽相同。 GmMNPV和TnMNPV的主要结构多肽在聚丙烯酰胺凝胶中具有与AcMNPV结构多肽相同的模式。与 AcMNPV 相比，GmMNPV 和 TnMNPV 有几个细微的结构蛋白差异。 AcMNPV 变种、TnMNPV 和 GmMNPV 不同，但具有非常相似的基因组和蛋白质结构。
The restriction sites of Autographa californica nuclear polyhedrosis virus (AcMNPV) E2 DNA were mapped for the endonucleases SmaI, KpnI, BamHI, SacI, XhoI, and EcoRI. The restriction maps of four other AcMNPV variants, Trichoplusia ni (TnMNPV), and Galleria mellonella (GmMNPV) genomes were determined and compared to the endonuclease cleavage maps of AcMNPV E2 DNA. The viral structural polypeptides of AcMNPV variants S3, E2, S1, M3, and R9 were the same when analyzed by polyacrylamide gel electrophoresis. The major structural polypeptides of GmMNPV and TnMNPV had the same pattern in polyacrylamide gels as did AcMNPV structural polypeptides. GmMNPV and TnMNPV had several minor structural protein differences as compared with AcMNPV. AcMNPV variants, TnMNPV, and GmMNPV were distinct but with very similar genomes and protein structures.