THE NUTRITION OF THE LARVA OF AEDES-AEGYPTI LINNAEUS .4. PROTEIN AND AMINO-ACID REQUIREMENTS
THE NUTRITION OF THE LARVA OF AEDES-AEGYPTI LINNAEUS .4. PROTEIN AND AMINO-ACID REQUIREMENTS
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DOI：
10.1042/bj0430379
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发表时间：
1948-01-01
影响因子：
4.1
通讯作者：
DEMEILLON, B
中科院分区：
文献类型：
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作者：
GOLBERG, L;DEMEILLON, B
在其他方面完全但缺乏蛋白质的培养基中，蚊子幼虫不能生长到2龄。除了酵母，酪蛋白是最合适的蛋白质，但不完全蛋白质，补充氨基酸，证明足以生长和存活到成虫阶段。通过使用氨基酸的混合物可以产生良好的生长率和存活率，但是它们的相对比例的变化或氨基酸数量的减少具有不利影响。通过从混合物中省略单个氨基酸，可以确定以下是蚊子幼虫所必需的：甘氨酸、L-亮氨酸、DL-异亮氨酸、L-组氨酸、L-精氨酸、L-赖氨酸、L-色氨酸、DL-苏氨酸、DL-苯丙氨酸和DL-甲硫氨酸。在培养基中使用酵母自溶物时，无法测定DL-缬氨酸的状态。有证据表明，L-胱氨酸的缺失导致很高比例的成年蚊子未能出现。根据培养基中苯丙氨酸或酪氨酸的水平，蚊幼虫可产生各种深浅的色素沉着。即使从完全无色素的幼虫中出现的成虫也是正常色素沉着的，色素沉着与生长或存活之间似乎没有什么关系。发现许多化合物能够在蚊子幼虫中产生中度色素沉着。
In a medium complete in other respects, but lacking protein,the mosquito larva does not grow to the 2d instar. After yeast, casein is the most suitable protein, but incomplete proteins, on supplementation with amino acids, prove adequate for growth and survival to the adult stage. Good rates of growth and survival could be produced by using mixtures of amino acids, but variation in their relative proportions or reduction in the number of amino acids had adverse effects. By omitting single amino acids from a mixture, it was possible to establish that the following are essential for the mosquito larva; glycine, L-leucine, DL-isoleucine, L-histidine, L-arginine, L-lysine, L-tryptophan, DL-threonine, DL-phenylalanine and DL-methionine. The status of DL-valine could not be determined while using yeast autolysate in the medium. There was evidence that the omission of L-cystine resulted in a high proportion of adult mosquitoes failing to emerge. According to the level of phenylalanine or tyrosine in the medium various shades of pigmentation could be produced in the mosquito larvae. The adults emerging even from wholly unpigmented larvae were normally pigmented and there appeared to be little relation between pigmentation and growth or survival. A number of compounds were found capable of producing intermediate degrees of pigmentation in the mosquito larva.