Polymorphism of Kdo-Based Glycolipids: The Elaborately Determined Stable and Dynamic Bicelles
Polymorphism of Kdo-Based Glycolipids: The Elaborately Determined Stable and Dynamic Bicelles
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Kdo 基糖脂的多态性：精心确定的稳定和动态 Bicelles
DOI：
10.31635/ccschem.021.202101168
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发表时间：
2022-07-01
期刊：
影响因子：
11.2
通讯作者：
Chen, Guosong
中科院分区：
文献类型：
--
作者：
Feng, Yingle;Li, Long;Chen, Guosong
了解糖脂的多样性，包括其化学结构和组成如何影响其生物学功能，是一个显着的基本挑战。本论文以一种稀有的单糖3-脱氧-D-甘露-2-辛酮糖酸（Kdo）为研究对象，建立了一个简单的仿生模型，从超分子化学的角度来理解糖脂的多样性。选择Kdo不仅是因为其不寻常的8碳酸性碳水化合物主链与普通的己糖非常不同，还因为其在脂多糖中的关键结构作用以及在细菌，植物和藻类中的流行。发现尽管两种Kdo-脂质S-Kdo和Kdo-S均衍生自相同的碳水化合物主链并且作为其自组装形态产生了双胞，但实验结果揭示了自组装显示出途径复杂性。Bicelle是S-Kdo的热力学产物，而对于Kdo-S，Bicelle仅是动力学捕获态，其最终转变为带。分子模拟清楚地揭示了Kdo-脂质在双胞中的不同包装，具有来自氢键和静电相互作用的不同贡献。[图形]
Understanding how the diversity of glycolipids, including how their chemical structures and composition affect their biological functions, is a remarkable fundamental challenge. In this work, we employed a rare monosaccharide, 3-deoxy-D-manno-2-octulosonic acid (Kdo) to build a simple and biomimetic model to understand the diversity of glycolipids from the viewpoint of supramolecular chemistry. Kdo was chosen not only because its unusual 8-carbon acidic carbohydrate backbone is very different from common hexoses, but also because of its key structural role in lipopolysaccharides and prevalence in bacteria, plant life, and algae. It was found that although both of the two Kdo-lipids S-Kdo and Kdo-S derived from the same carbohydrate backbone and gave bicelles as their self- assembled morphology, experimental results revealed that the self-assembly showed pathway complexity. Bicelle is the thermodynamic product of S-Kdo, while for Kdo-S, the bicelle is only a kinetically trapped state, which finally transforms to a ribbon. Molecular simulation clearly revealed the different packing of Kdo-lipids in the bicelles with different contribution from hydrogen bonds and electrostatic interactions.[GRAPHICS]