Preparation of a hydroxypropyl Ganoderma lucidum polysaccharide and its physicochemical properties
Preparation of a hydroxypropyl Ganoderma lucidum polysaccharide and its physicochemical properties
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羟丙基灵芝多糖的制备及其理化性质
DOI：
10.1016/j.foodchem.2009.11.087
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发表时间：
2010-10-15
期刊：
影响因子：
8.8
通讯作者：
Yu, Liangli (Lucy)
中科院分区：
文献类型：
--
作者：
Liu, Wei;Xu, Jing;Yu, Liangli (Lucy)
以低值水不溶性灵芝多糖(GLP)为原料，采用环氧丙烷在碱性条件下制备羟丙基灵芝多糖(H-GLP)。 H-GLP 通过 IR 和 C-13 NMR 光谱表征其化学结构，并分析其单糖组成、分子量和羟丙基含量。 H-GLP含有甘露糖、葡萄糖和半乳糖，摩尔比分别为1.0:36.5:3.59，平均分子量为788 kDa，羟丙基含量为12.05%。 H-GLP 还具有超过 50 mg/mL 的优异水溶性，表明羟丙基化可能是提高 GLP 水溶性的有效方法。还比较了 H-GLP 与原始 GLP 和抗坏血酸的抗氧化特性。 H-GLP 对羟基、超氧阴离子自由基和过氧化氢的自由基清除能力比 GLP 强得多。这些结果表明羟丙基化在从 GLP 中开发水溶性抗氧化多糖以提高灵芝生产和加工行业的盈利能力方面具有潜力。 (C) 2010 Elsevier Ltd. 保留所有权利。
A hydroxypropyl Ganoderma lucidum polysaccharide (H-GLP) was prepared from a low-value water-insoluble polysaccharide from G. lucidum (GLP) using propylene oxide under an alkaline condition. The H-GLP was characterised for its chemical structure with IR and C-13 NMR spectra and analysed for its mono-sugar composition, molecular weight, and hydroxypropyl content. H-GLP contained mannose, glucose, and galactose in a mole ratio of 1.0:36.5:3.59, respectively, with an average molecular weight of 788 kDa and a hydroxypropyl content of 12.05%. H-GLP also had an excellent water solubility of more than 50 mg/mL, suggesting that hydroxypropylation might serve as an effective approach to enhance water solubility of GLP. H-GLP was also compared to the original GLP and ascorbic acid for antioxidant properties. H-GLP showed much stronger free radical scavenging capacity against hydroxyl and superoxide anion radicals and hydrogen peroxide than GLP. These results suggested the potential of hydroxypropylation in developing water-soluble antioxidative polysaccharides from GLP to enhance the profitability of G. lucidium production and processing industries. (C) 2010 Elsevier Ltd. All rights reserved.