Additive assay of cancer marker CA 19-9 by SPR biosensor
Additive assay of cancer marker CA 19-9 by SPR biosensor
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DOI：
10.1016/j.snb.2006.04.015
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发表时间：
2006-10-25
影响因子：
8.4
通讯作者：
Pyun, J. C.
中科院分区：
文献类型：
--
作者：
Chung, J. W.;Bernhardt, R.;Pyun, J. C.
癌症标志物碳水化合物抗原（CA 19-9）的检测通过相加测定进行，该相加测定旨在使免疫亲和生物传感器在分析之间不进行任何化学处理的情况下测量样品。通过使用累积浓度和累积信号之间的相关曲线，从实际测量的信号计算连续注入的样品的每个浓度。根据CA 19-9的真实的浓度与计算浓度之间的比较，估计误差约为5%，计算出CA 19-9的检测限为410.9 U/ml。为提高CA 19-9检测的灵敏度，在每次进样后追加CA 19-9抗体形成夹心复合物，检测限提高到66.7U/ml。(c)2006 Elsevier B. V.保留所有权利。
The detection of a cancer marker, carbohydrate antigen (CA 19-9) was performed by the additive assay which aims for immunoaffinity biosensors to measure the samples without any chemical treatment between analyses. Each concentration of the continually injected sample was calculated from the actually measured signal by using the correlation curve between the accumulated concentration and the accumulated signal. From the comparison between the real and the calculated concentration of CA 19-9, the error was estimated to be around 5% and the detection limit of CA 19-9 was calculated to be 410.9 U/ml. In order to improve the sensitivity of CA 19-9 detection, the antibodies against CA 19-9 was additionally injected after each sample injection for the formation of sandwich complex, and the detection limit was improved to be 66.7 U/ml. (c) 2006 Elsevier B.V. All rights reserved.