Transfer of high-mannose-type oligosaccharides to disaccharides by endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae
Transfer of high-mannose-type oligosaccharides to disaccharides by endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae
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DOI：
10.1263/jbb.93.614
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发表时间：
2002-06-01
影响因子：
2.8
通讯作者：
Takegawa, K
中科院分区：
文献类型：
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作者：
Fujita, K;Miyamura, T;Takegawa, K
来自原节杆菌 (Endo-A) 的内切-β-N-乙酰氨基葡萄糖苷酶具有转糖基活性，可将高甘露糖型寡糖转移至合适的糖苷作为受体底物。研究了 Endo-A 催化的转糖基化对各种二糖的受体特异性。为了鉴定 Endo-A 转糖基化的有效受体，使用各种二糖进行反应。 Endo-A 将高甘露糖型寡糖更有效地转移到 P 连接二糖（纤维二糖、龙胆二糖、槐糖和昆布二糖）上，而不是转移到作为受体底物的 a 连接二糖（异麦芽糖、麦芽糖、黑曲二糖、曲二糖和海藻糖）上。转糖基化产物 (Man)(6)GlcNAc-Glc-beta-Glc 的水解速度比 (Man)(6)GlcNAc-Glc-α-Glc 更快。这些结果表明Endo-A识别受体底物的异头构型，并且P-连接糖苷适合于转糖基化产物的合成。
Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae (Endo-A) has transglycosylation activity, and high-mannose-type oligosaccharides are transferred to suitable glycosides as acceptor substrates. The acceptor specificity of Endo-A-catalyzed transglycosylation toward various disaccharides was investigated. To identify an effective acceptor for the transglycosylation by Endo-A, the reaction was carried out using various disaccharides. Endo-A transferred high-mannose-type oligosaccharides more efficiently to P-linked disaccharides (cellobiose, gentiobiose, sophorose, and laminaribiose) than to a-linked disaccharides (isomaltose, maltose, nigerose, kojibiose, and trehalose) as acceptor substrates. The transglycosylation products, (Man)(6)GlcNAc-Glc-beta-Glc, were more rapidly hydrolyzed than (Man)(6)GlcNAc-Glc-alpha-Glc. These results indicate that Endo-A recognizes the anomeric configuration of the acceptor substrates, and P-linked glycosides are suitable for the synthesis of transglycosylation products.