Primary tumor microRNA signature predicts recurrence and survival in patients with locally advanced esophageal adenocarcinoma.
Primary tumor microRNA signature predicts recurrence and survival in patients with locally advanced esophageal adenocarcinoma.
复制标题
DOI：
10.18632/oncotarget.12832
复制
发表时间：
2016-12-06
期刊：
影响因子：
--
通讯作者：
Jobe BA
中科院分区：
文献类型：
--
作者：
Matsui D;Zaidi AH;Martin SA;Omstead AN;Kosovec JE;Huleihel L;Saldin LT;DiCarlo C;Silverman JF;Hoppo T;Finley GG;Badylak SF;Kelly RJ;Jobe BA
食管腺癌（EAC）是一种侵袭性癌症，需要开发改进的风险分层工具来进行个性化护理。此前，microRNA已被证明与各种癌症类型的进展和预后相关。然而，EAC 的价值在很大程度上仍未被开发。我们对 32 个福尔马林固定、石蜡包埋的 EAC 标本进行了整体 microRNA 分析，以鉴定与进展相关的 microRNA。文献检索和通路分析进一步细化了五个显着放松管制的候选生物标志物的输出。通过 qRT-PCR 验证了 5 种 microRNA 中的 4 种（miR-652-5p、miR-7-2-3p、miR-3925-3p 和 miR-219-3p）。在 26 名 II/III 期 EAC 患者的测试组中评估生存结果，以确定所选 microRNA 的预后相关性。在测试集中，miR-652-5p 和 miR-7-2-3p 表达与无进展生存期显着相关（p 值 = .00771 和 p 值 = .00293）。 miR-652-5p 和 miR-7-2-3p 组合的受试者工作特征 (ROC) 曲线下面积最高为 0.8212。总的来说，我们的研究结果表明，miR-652-5p/miR-7-2-3p 特征可能作为局部晚期 EAC 患者的一个有希望的预后标志物。
Esophageal adenocarcinoma (EAC) is an aggressive cancer necessitating the development of improved risk stratification tools for personalized care. Previously, microRNAs have been shown to correlate with the progression and prognosis of various cancer types; however, the value in EAC remains largely unexplored. We performed global microRNA profiling on 32 formalin-fixed, paraffin-embedded EAC specimens to identify microRNAs associated with progression. Literature search and pathway analysis further refined output to five significantly deregulated candidate biomarkers. Four of the five microRNAs (miR-652-5p, miR-7-2-3p, miR-3925-3p, and miR-219-3p) were validated by qRT-PCR. Survival outcomes were evaluated in testing set of 26 stage II/III EAC patients to determine the prognostic relevance of the selected microRNAs. In the testing set, miR-652-5p and miR-7-2-3p expressions were significantly associated with progression-free survival (p-value = .00771 and p-value = .00293). The highest area under receiver operating characteristic (ROC) curve was 0.8212 for the combination of miR-652-5p and miR-7-2-3p. Collectively, our findings demonstrated that the miR-652-5p/miR-7-2-3p signature may serve as a promising prognostic marker in patients with locally advanced EAC.