Slug is a novel downstream target of MyoD - Temporal profiling in muscle regeneration
Slug is a novel downstream target of MyoD - Temporal profiling in muscle regeneration
复制标题
DOI：
10.1074/jbc.m202668200
复制
发表时间：
2002-08-16
影响因子：
4.8
通讯作者：
Hoffman, EP
中科院分区：
文献类型：
--
作者：
Zhao, P;Iezzi, S;Hoffman, EP
利用时间表达谱来定义小鼠肌肉再生模型中的体内转录调控途径。通过时间表达、启动子数据库挖掘和凝胶迁移分析鉴定了MyoD的潜在下游靶点; Slug和钙蛋白酶6被鉴定为新型MyoD靶点。Slug是对中胚层/外胚层发育至关重要的锌指转录抑制子的蜗牛/slug家族的成员，通过使用启动子/报告基因构建体和Slug缺失小鼠中有缺陷的肌肉再生的证明，进一步显示Slug是下游靶标。
Temporal expression profiling was utilized to define transcriptional regulatory pathways in vivo in a mouse muscle regeneration model. Potential downstream targets of MyoD were identified by temporal expression, promoter data base mining, and gel shift assays; Slug and calpain 6 were identified as novel MyoD targets. Slug, a member of the snail/slug family of zinc finger transcriptional repressors critical for mesoderm/ectoderm development, was further shown to be a downstream target by using promoter/reporter constructs and demonstration of defective muscle regeneration in Slug null mice.