The potyvirus recessive resistance gene, sbm1, identifies a novel role for translation initiation factor eIF4E in cell-to-cell trafficking
The potyvirus recessive resistance gene, sbm1, identifies a novel role for translation initiation factor eIF4E in cell-to-cell trafficking
复制标题
DOI：
10.1111/j.1365-313x.2004.02215.x
复制
发表时间：
2004-11-01
期刊：
影响因子：
7.2
通讯作者：
Maule, AJ
中科院分区：
文献类型：
--
作者：
Gao, ZH;Johansen, E;Maule, AJ
从豌豆中的隐性抗性基因sbm 1的特性，我们已经确定了真核翻译起始因子，eIF 4 E，作为感染马铃薯Y病毒，豌豆种子传播的花叶病毒所需的易感因子。显性等位基因产物的作用模式的功能分析揭示了eIF 4 E在其支持病毒从细胞到细胞的移动中的新功能，除了其可能支持病毒RNA翻译，因此复制。在两个独立的豌豆系不同的抗性特异性解释了eIF 4 E的不同突变。在eIF 4 E的模型结构上，两种情况下的编码变化都位于与真核mRNA的5 '-m（7）G帽结合有关的结构口袋中和周围。蛋白质表达和帽结合分析表明，抗性植物编码的eIF 4 E不能与m（7）G-Sepharose结合，这一结果可能表明抗性豌豆中eIF 4 E和旁系同源eIF（iso）4 E之间存在功能冗余。这些观察结果，以及其他马铃薯Y病毒隐性抗性的相关发现，提供了马铃薯Y病毒生命周期的更完整的图片。
From the characterization of the recessive resistance gene, sbm1, in pea we have identified the eukaryotic translation initiation factor, eIF4E, as a susceptibility factor required for infection with the Potyvirus, Pea seed-borne mosaic virus. A functional analysis of the mode of action of the product of the dominant allele revealed a novel function for eIF4E in its support for virus movement from cell-to-cell, in addition to its probable support for viral RNA translation, and hence replication. Different resistance specificities in two independent pea lines were explained by different mutations in eIF4E. On the modelled structure of eIF4E the coding changes were in both cases lying in and around the structural pocket involved in binding the 5'-m(7)G cap of eukaryotic mRNAs. Protein expression and cap-binding analysis showed that eIF4E encoded by a resistant plant could not bind to m(7)G-Sepharose, a result which may point to functional redundancy between eIF4E and the paralogous eIF(iso)4E in resistant peas. These observations, together with related findings for other potyvirus recessive resistances, provide a more complete picture of the potyvirus life cycle.