Validation and application of a rapid in vitro assay for assessing the estrogenic potency of halogenated phenolic chemicals
Validation and application of a rapid in vitro assay for assessing the estrogenic potency of halogenated phenolic chemicals
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DOI：
10.1016/s0045-6535(98)00297-5
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发表时间：
1998-10-01
期刊：
影响因子：
8.8
通讯作者：
Hagenmaier, H
中科院分区：
文献类型：
--
作者：
Korner, W;Hanf, V;Hagenmaier, H
E-Screen检测试剂盒是一种体外工具，用于检测化学品和环境样本提取物的雌激素活性。基于人雌激素受体阳性MCF-7乳腺癌细胞增殖的诱导，我们可以大大简化测定。作为验证的一个重要步骤，我们应用改进的测定法检测9种已知的异种雌激素。我们可以确认其他组的结果，确保E-Screen检测的重现性。结果提供了证据表明，E-Screen测定法适用于测定环境雌激素的雌二醇等效因子（EEFs），以相对于天然雌激素17 β-雌二醇对其雌激素效力进行排名。此外，我们使用了优化的增殖试验，以筛选9卤代酚类化合物，其可能的雌激素效力。三种广泛应用的化学物质表现出弱的受体介导的雌激素活性：阻燃剂四溴双酚A，消毒剂4-氯-3-甲基苯酚，和除草剂离析物4-氯-2-甲基苯酚。它们的雌激素效力比17 β-雌二醇低5至6个数量级。（C）1998爱思唯尔科技有限公司版权所有
The E-Screen assay serves as an in vitro tool for the detection of estrogenic activity of chemicals and extracts of environmental samples. Based on the induction of proliferation in human estrogen receptor-positive MCF-7 breast cancer cells we could substantially simplify the assay. As one important step of validation we applied the modified assay for testing nine known xenoestrogens. We could confirm the results of other groups assuring the reproducibility of the E-Screen assay. The results provide evidence that the E-Screen assay is suitable for determination of estradiol equivalency factors (EEFs) for environmental estrogens to rank their estrogenic potency relative to the natural estrogen 17 beta-estradiol. Further, we used the optimized proliferation test to screen nine halogenated phenolic compounds for their possible estrogenic potency. Three widely applied chemicals expressed a weak receptor-mediated estrogenic activity: the flame retardant Tetrabromo-Bisphenol-A, the disinfectant 4-chloro-3-methylphenol, and the herbicide educt 4-chloro-2-methylphenol. Their estrogenic potencies were five to six orders of magnitude lower than that of 17 beta-estradiol. (C)1998 Elsevier Science Ltd. All rights reserved