Multiple competition reactions for RPA order the assembly of the DNA polymerase δ holoenzyme
Multiple competition reactions for RPA order the assembly of the DNA polymerase δ holoenzyme
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DOI：
10.1093/emboj/18.21.6189
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发表时间：
1999-11-01
期刊：
影响因子：
11.4
通讯作者：
O'Donnell, M
中科院分区：
文献类型：
--
作者：
Yuzhakov, A;Kelman, Z;O'Donnell, M
真核生物中 DNA 的持续延伸需要三个因素来协调其作用，首先，DNA 聚合酶 α-引发酶合成引发位点。然后复制因子 C 将增殖细胞核抗原 (PCNA) 夹加载到引物上。此后，DNA 聚合酶 delta 与 PCNA 组装以进行持续延伸。该报告表明，这些蛋白质均与引物位点紧密结合，并以高度协调的方式交换位置，使得引物末端永远不会没有蛋白质。复制蛋白 A (RPA) 是一种单链 DNA 结合蛋白，为这些蛋白质中的每一种形成一个共同的接触点，并且它们相互竞争。因此，这些蛋白质交换是由基于竞争的蛋白质开关驱动的，其中两种蛋白质竞争与 RPA 的接触。
Processive extension of DNA in eukaryotes requires three factors to coordinate their actions, First, DNA polymerase alpha-primase synthesizes the primed site. Then replication factor C loads a proliferating cell nuclear antigen (PCNA) clamp onto the primer. Following this, DNA polymerase delta assembles with PCNA for processive extension. This report shows that these proteins each bind the primed site tightly and trade places in a highly coordinated fashion such that the primer terminus is never left free of protein. Replication protein A (RPA), the single-stranded DNA-binding protein, forms a common touchpoint for each of these proteins and they compete with one another for it. Thus these protein exchanges are driven by competition-based protein switches in which two proteins vie for contact with RPA.