Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes
Effect of transforming growth factor-β3 on the expression of Smad3 and Smad7 in tenocytes
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DOI：
10.3892/mmr.2016.4944
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发表时间：
2016-04-01
影响因子：
3.4
通讯作者：
Xiong, Yan
中科院分区：
文献类型：
--
作者：
Jiang, Ke;Chun, Guo;Xiong, Yan
肌腱粘连是肌腱损伤愈合过程中常见的问题。TGF-β/Smad信号通路的分子机制已经确定，并且TGF-β在伤口愈合中的作用已经得到很好的表征。然而，TGF-β 3调节其对肌腱愈合作用的细胞内机制或下游信号尚未得到很好的阐明。本研究的目的是确定TGF-β 3对肌腱细胞中TGF-β/Smad信号通路的影响。采用定量聚合酶链反应（PCR）和Western blot分析TGF-β 3对肌腱细胞Smad蛋白表达的调节作用。结果表明，TGF-β 3对肌腱细胞的增殖无明显影响。TGF-β 3能在基因和蛋白水平上显著下调Smad 3的表达，上调Smad 7的表达。结果表明，TGF-β 3可以通过TGF-β/Smad信号通路调节Smad 3和Smad 7蛋白，以最小化外源性瘢痕形成。因此，它可能提供一种新的方法来减少肌腱粘连，促进肌腱愈合。
Tendon adhesion is a common problem in the healing of injured tendons. The molecular mechanisms of the TGF-beta/Smad signaling pathway have been determined, and the role of TGF-beta has been well characterized in wound healing. However, the intracellular mechanism or downstream signals by which TGF-beta 3 modulates its effects on tendon healing have not been well elucidated. The aim of this study was to determine the effect of TGF-beta 3 on the TGF-beta/Smad signaling pathway in tenocytes. Quantitative polymerase chain reaction and western blot analysis were used to analyze the effect of TGF-beta 3 on the regulation of the expression of Smad proteins in tenocytes. The results demonstrated that TGF-beta 3 has no significant effect on the proliferation of tendon cells. The addition of TGF-beta 3 to tenocytes can significantly downregulate the expression of Smad3 and upregulate the expression of Smad7 at the gene and protein levels. The results demonstrate that TGF-beta 3 may regulate Smad3 and Smad7 proteins through the TGF-beta/Smad signaling pathway to minimize extrinsic scarring. Thus, it may provide a novel approach to decrease tendon adhesion and promote tendon healing.