Programmable bio-nano-chip system: a flexible point-of-care platform for bioscience and clinical measurements.
Programmable bio-nano-chip system: a flexible point-of-care platform for bioscience and clinical measurements.
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DOI：
10.1039/c5lc00636h
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发表时间：
2015-10-21
期刊：
影响因子：
6.1
通讯作者：
McDevitt JT
中科院分区：
文献类型：
--
作者：
McRae MP;Simmons GW;Wong J;Shadfan B;Gopalkrishnan S;Christodoulides N;McDevitt JT
开发用于通用生物检测系统的集成仪器是芯片实验室社区的一个关键目标。可编程生物纳米芯片(p-BNC)系统是一种用于生物科学和临床测量的多功能、多路和多类别的化学和生物传感系统。该系统由两个主要部件组成，一个是一次性墨盒，另一个是便携式分析仪。可定制的一次性塑料墨盒现在可以通过注塑大量生产，其设计用于分析性能、易用性、重复性和低成本。这些LabCard设备实现了高表面积纳米结构生物标记物捕获元件，能够实现高性能的信号传递，并与真实世界的生物样本进行索引匹配。这种检测方式，加上方便地在水泡和自备废物中存储芯片上的液体，代表了在护理地点数字化生物签名的标准过程。一种配套的便携式分析仪原型已经开发出来，它集成了流体激励、光学检测和自动数据分析，并作为完全分析自动化的人机界面。在这份报告中，我们提供了p-BNC通用生物传感平台的系统级视角，重点是流量控制、设备集成和自动化。为了证明p-BNC的灵活性，我们通过三种重要的疾病应用区分疾病患者和非患者：前列腺癌、卵巢癌和急性心肌梗死。介绍了使用全自动p-BNC系统开发快速7分钟肌红蛋白分析的进展。
The development of integrated instrumentation for universal bioassay systems serves as a key goal for the lab-on-a-chip community. The programmable bio-nano-chip (p-BNC) system is a versatile multiplexed and multiclass chemical- and bio-sensing system for bioscience and clinical measurements. The system is comprised of two main components, a disposable cartridge and a portable analyzer. The customizable single-use plastic cartridges, which now can be manufactured in high volumes using injection molding, are designed for analytical performance, ease of use, reproducibility, and low cost. These labcard devices implement high surface area nano-structured biomarker capture elements that enable high performance signaling and are index matched to real-world biological specimens. This detection modality, along with the convenience of on-chip fluid storage in blisters and self-contained waste, represents a standard process to digitize biological signatures at the point-of-care. A companion portable analyzer prototype has been developed to integrate fluid motivation, optical detection, and automated data analysis, and it serves as the human interface for complete assay automation. In this report, we provide a systems-level perspective of the p-BNC universal biosensing platform with an emphasis on flow control, device integration, and automation. To demonstrate the flexibility of the p-BNC, we distinguish diseased and non-case patients across three significant disease applications: prostate cancer, ovarian cancer, and acute myocardial infarction. Progress towards developing a rapid 7 minute myoglobin assay is presented using the fully automated p-BNC system.