Circular RNAs Hsa_circ_0002715 and Hsa_circ_0035197 in Peripheral Blood Are Novel Potential Biomarkers for New-Onset Rheumatoid Arthritis
Circular RNAs Hsa_circ_0002715 and Hsa_circ_0035197 in Peripheral Blood Are Novel Potential Biomarkers for New-Onset Rheumatoid Arthritis
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外周血中的环状 RNA Hsa_circ_0002715 和 Hsa_circ_0035197 是新发类风湿关节炎的新型潜在生物标志物
DOI：
10.1155/2019/2073139
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发表时间：
2019-10-29
期刊：
影响因子：
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通讯作者：
Li, Junmin
中科院分区：
文献类型：
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作者：
Luo, Qing;Liu, Jun;Li, Junmin
本研究旨在探讨外周血循环RNA(CircRNAs)水平作为新发类风湿性关节炎(RA)诊断的候选生物标志物。用定量逆转录聚合酶链式反应(qRT-PCR)检测22例初发RA患者和健康对照(HC)外周血中CircRNA的含量。新发RA患者外周血中hSA_CIRC_0002715、hSA_CIRC_0001947、hSA_CIRC_0000367和hSA_CIRC_0035197水平显著高于HC患者。新发RA患者与系统性红斑狼疮(SLE)、强直性脊柱炎(AS)患者外周血上述四项指标均有明显差异。新发RA患者与未确诊关节炎(UA)患者的hSA_CIRC_0001947、hSA_CIRC_0035197有显著差异。受试者工作特征(ROC)曲线分析提示，外周血hSA_CIRC_0002715和hSA_CIRC_0000367水平可区分新发RA患者与HC、AS、SLE患者；外周血HSA_CIRC_0001947、0035197水平可区分新发RA患者与HC、AS、SLE、UA患者。Logistic回归模型显示，hSA_CIRC_0002715和hSA_CIRC_0035197联合检测的诊断准确率最高，曲线下面积为0.758(敏感性：72.9%，特异性：71.4%)。此外，外周血hsA_CIRC_0002715水平与关节肿胀数、关节压痛数、病程、类风湿因子、抗核蛋白抗体、血液学紊乱相关。外周血HSA_CIRC_0035197水平与血液病相关。本研究提示外周血中hSA_CIRC_0002715和hSA_CIRC_0035197的联合检测可能是新发类风湿关节炎患者潜在的生物标志物，并可能与疾病活动性有关。
This study is aimed at exploring the levels of peripheral blood circular RNAs (circRNAs) as biomarker candidates for the diagnosis of new-onset rheumatoid arthritis (RA). The selected twenty-two circRNAs in peripheral blood from new-onset RA patients and healthy controls (HC) were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The levels of hsa_circ_0002715, hsa_circ_0001947, hsa_circ_0000367, and hsa_circ_0035197 were significantly increased in the peripheral blood of new-onset RA patients than in the peripheral blood of HC. And, there were obvious differences in the above four peripheral blood circRNAs between new-onset RA patients and systemic lupus erythematosus (SLE) patients and ankylosing spondylitis (AS) patients. Moreover, there were obvious differences in hsa_circ_0001947 and hsa_circ_0035197 between new-onset RA patients and patients with undiagnosed arthritis (UA). Receiver operating characteristic (ROC) curve analysis suggested that the levels of hsa_circ_0002715 and hsa_circ_0000367 in peripheral blood could distinguish new-onset RA patients from the HC, AS patients, and SLE patients, and the levels of hsa_circ_0001947 and hsa_circ_0035197 in peripheral blood could distinguish new-onset RA patients from the HC, AS patients, SLE patients, and UA patients. The logistic regression model showed that the combination of hsa_circ_0002715 and hsa_circ_0035197 could provide the best diagnostic accuracy with an area under the curve (AUC) of 0.758 (sensitivity: 72.9%, specificity: 71.4%). Moreover, the levels of peripheral blood hsa_circ_0002715 were correlated with swollen joint count (SJC), tender joint count (TJC), disease duration, rheumatoid factor (RF), anticitrullinated protein antibodies (ACPA), and hematologic disorder. And, the levels of peripheral blood hsa_circ_0035197 were correlated with hematologic disorder. This study suggests that the combination of hsa_circ_0002715 and hsa_circ_0035197 in peripheral blood may be a potential biomarker of patients with new-onset RA and may be associated with disease activity.