The genetics of bone marrow transplantation in the rat.
The genetics of bone marrow transplantation in the rat.
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大鼠骨髓移植的遗传学。
DOI：
10.1097/00007890-198501000-00008
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发表时间：
1985
期刊：
影响因子：
6.2
通讯作者：
Cramer,DV
中科院分区：
文献类型：
--
作者：
Oaks,MK;Cramer,DV
我们已经使用了各种标准的近交系，重组，和同源大鼠品系，以建立骨髓移植的MHC和非MHC遗传不相容性的影响。这些位点在成功建立骨髓移植中的作用首先通过检查去核骨髓保护受体大鼠免受致死剂量的清髓药物白消安的潜力来确定。然后使用类似的供体/受体组合来检查相同的不相容性对用白消安和环磷酰胺组合调节的受体中的致命性移植物抗宿主病（GVHD）的诱导的影响。获得的数据表明：（1）大鼠整个MHC的差异足以防止骨髓移植并产生致命的GVHD;（2）I类RT 1。大鼠MHC基因座对骨髓移植有不同的影响，因为该基因座的差异会阻止成功的骨髓移植，而该基因对致命性GVHD的发展几乎没有影响;（3）I类RT 1的差异。E基因座对骨髓移植无影响，不刺激GVHD;（4）II类基因座RT 1的差异。非MHC基因的不相容性可阻止骨髓植入的建立并引发致死性GVHD。
We have used a variety of standard inbred, recombinant, and congenic rat strains to establish the effect of MHC and non-MHC genetic incompatibilities on bone marrow transplantation. The role of these loci in the successful establishment of bone marrow engraftment was first determined by examining the potential of do-nor marrow to protect recipient rats from a lethal dose of the myeloablative drug busulfan. Similar donor/recipient combinations were then used to examine the effects of the same incompatibilities on the induction of fatal graft-versus-host disease (GVHD) in recipients conditioned with a combination of busulfan and cyclophosphamide. The data obtained indicate that:(1) a difference for the entire MHC of the rat is sufficient to prevent marrow engraftment and to produce fatal GVHD;(2) the class I RT1. A locus of the rat MHC has a differential effect on bone marrow transplantation, since disparity for this locus prevents successful marrow engraftment, while this gene has little effect on the development of fatal GVHD;(3) disparity for the class I RT1. E locus has no effect on bone marrow engraftment and does not stimulate GVHD;(4) disparity for the class II locus, RT1. D, can prevent marrow engraftment and elicit fatal GVHD; and (5) incompatibility for non-MHC genes can prevent the establishment of bone marrow engraftment and elicit fatal GVHD.