A NEURAL DEGENERATION MUTATION THAT SPARES PRIMARY NEURONS IN THE ZEBRAFISH
A NEURAL DEGENERATION MUTATION THAT SPARES PRIMARY NEURONS IN THE ZEBRAFISH
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DOI：
10.1016/0012-1606(88)90245-x
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发表时间：
1988-03-01
影响因子：
2.7
通讯作者：
STREISINGER, G
中科院分区：
文献类型：
--
作者：
GRUNWALD, DJ;KIMMEL, CB;STREISINGER, G
我们描述了斑马鱼Brachydanio rerio中的胚胎致命突变，该突变专门影响胚胎中枢神经系统（CNS）中大多数细胞的生存能力。用.gamma-irradiation诱导突变NED-1（B39RL），并将其分为单个隐性等位基因，与其中心粒密切相关。它在中枢神经系统中产生了巨大的细胞死亡，但是一小部分特定的神经元，包括Rohon-Beard感觉神经元，大的后脑中间神经元和原发性膜神经元，可在胚胎发生中存活并具有功能性。胚胎运动神经元和肌肉细胞之间的突触连接在生理上似乎正常，并且通常观察到的自发屈曲存在。与感觉神经元和中间神经元的存在相关，突变胚胎响应于机械刺激而表现出反射运动。一起，幸存的神经元称为原发性神经元，形成了一类大小突出并在发育过程中出现的细胞。因此，该突变可以定义一个函数，该函数是通过在胚胎中枢神经系统中可开发区分的细胞集差异所需的。
We describe an embryonic lethal mutation in the zebrafish Brachydanio rerio that specifically affects the viability of most cells in the embryonic central nervous system (CNS). The mutation ned-1 (b39rl) was induced with .gamma.-irradiation and segregates as a single recessive allele closely linked to its centromere. It produces massive cell death in the CNS but a small set of specific neurons, including Rohon-Beard sensory neurons, large hindbrain interneurons, and primary mononeurons, survive embryogenesis and are functional. Synaptic connections between embryonic motoneurons and muscle cells appear physiologically normal, and the normally observed spontaneous flexions are present. Correlated with the presence of sensory neurons and interneurons, mutant embryos display reflexive movements in response to mechanical stimulation. Together, the surviving neurons, called primary neurons, form a class of cells that are prominent in size and arise during development. Thus, this mutation may define a function that is differentially required by developmentally distinguishable sets of cells in the embryonic CNS.