Novel lap test determines the mechanics of delamination between annular lamellae of the intervertebral disc
Novel lap test determines the mechanics of delamination between annular lamellae of the intervertebral disc
复制标题
DOI：
10.1016/j.jbiomech.2010.08.031
复制
发表时间：
2011-01-04
影响因子：
2.4
通讯作者：
Callaghan, Jack P.
中科院分区：
文献类型：
--
作者：
Gregory, Diane E.;Veldhuis, Jim H.;Callaghan, Jack P.
纤维环层间脱层是椎间盘突出的关键阶段，为了更好地了解脱层过程的力学机制，设计了一种新的搭接试验。从冷冻猪脊柱的颈部区域获得由两个相邻的自然结合的椎板组成的标本。将它们切成标称3.5 mm宽× 7 mm长的样本，并从样本的相对端去除深层和浅层的翼片，以便在椎板之间保留4.5-5.0 mm长的完整界面。使用具有5个并排尖锐点的BioRake附件将样本安装在BioTester拉伸仪器中，并以2%/s应变。获得了力-时间曲线，并使用跟踪软件绘制了样本内位移的时间过程的详细地图。结果发现，层片本身的可扩展性使数据的解释大大复杂化。实验结果，结合数学分析和有限元模型，表明大部分剪切载荷是在粘结区的端部在薄板之间传递的，这一发现具有临床意义。发现层间粘合的峰值强度为0.30 +/- 0.05 N/mm试样宽度（不要与搭接长度混淆），并且即使当层间相对位移高达10 mm时，也具有显著的承载能力。(C)2010爱思唯尔有限公司版权所有。
Delamination between lamellae of the annulus fibrosus is a crucial stage of intervertebral disc herniation, and to better understand the mechanics of the delamination process, a novel lap test was devised. Specimens consisting of two adjacent, naturally bonded lamellae were obtained from the cervical region of frozen porcine spines. They were cut into specimens nominally 3.5 mm wide by 7 mm long and tabs of the deep and superficial layers were removed from opposite ends of the specimens so that a 4.5-5.0 mm long intact interface remained between the lamellae. Specimens were mounted in a BioTester tensile instrument using BioRake attachments having 5 sharpened points side-by-side, and they were strained at 2%/s. Force-time curves were obtained and, using tracking software, a detailed map was made of the time course of the displacements within the specimens. Extensibility of the lamellae themselves was found to substantially complicate interpretation of the data. The experiments, together with mathematical analyses and finite element models, show that much of the shear load is transferred between lamellae at the ends of the bonded region, a finding of clinical importance. The inter-lamellae bond was found to have a peak strength of 0.30 +/- 0.05 N/mm of specimen width (not to be confused with lap length), and the remarkable ability to carry substantial load even when lamellae had displaced up to 10 mm relative to each other. (C) 2010 Elsevier Ltd. All rights reserved.