Isolation and functional characterization of the pheromone biosynthesis activating neuropeptide receptor of Chinese oak silkworm, Antheraea pernyi
Isolation and functional characterization of the pheromone biosynthesis activating neuropeptide receptor of Chinese oak silkworm, Antheraea pernyi
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柞蚕信息素生物合成激活神经肽受体的分离及功能表征
DOI：
10.1016/j.ijbiomac.2018.05.145
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发表时间：
2018-10-01
影响因子：
8.2
通讯作者：
Wei, Zhao-Jun
中科院分区：
文献类型：
--
作者：
Jiang, Li;Zhang, Fang;Wei, Zhao-Jun
昆虫信息素生物合成激活神经肽（PBAN）控制着雌成虫性信息素的合成和激活。本研究从柞蚕信息素腺体（AntpePBANR）中克隆了PBAN受体的全长cDNA。AntpePBANR具有G蛋白偶联受体（G protein coupled receptor，GPCR）的7个跨膜区，在系统发育树中与家蚕和烟草天蛾的PBANR关系密切。哺乳动物细胞系中表达的AntpePBANR由AntpePBAN以浓度依赖性方式产生。AntpePBANR激活导致钙动员，但不激活cAMP升高途径。表达AntpePBANR的细胞对Antpe-gamma-SGNP（suboesophageal ganglion neuropeptides）和Antpe-DH（diapause hormone）有强烈的反应，Antpe-DH是A. pernyi。C-末端六肽（FSPRL酰胺）中残基的缺失证明P、R和L在起始AntpePBANR中起关键作用，氨基化至最后的C末端残基，其同样也可以完全影响AntpePBAN受体的活化。AntpePBANR基因的mRNA在信息素腺体中的转录水平最高，其次是脂肪体。(C)2018 Elsevier B. V.版权所有。
Insect pheromone biosynthesis activating neuropeptide (PBAN) controls the synthesis and actuating of sex pheromones of female adult. In the current examination, the full-length cDNA encoding the PBAN receptor was cloned from the pheromone gland (PG) of Antheraea pernyi (AntpePBANR). The AntpePBANR displayed the characteristic seven transmembrane areas of the G protein-coupled receptor (GPCR) and was closely related to the PBANR from Bombyx mori and Manduca sexta in the phylogenetic tree. The AntpePBANR expressed in mammalian cell lines were enacted by AntpePBAN in a concentration-dependent manner. AntpePBANR activation resulted in the calcium mobilization but did not activate the cAMP elevation pathway. Cells expressing AntpePBANR were profoundly responsive to Antpe-gamma-SGNP (suboesophageal ganglion neuropeptides) and Antpe-DH (diapause hormone), different individuals from FXPRLamide (X = T, S or V) family in A. pernyi. Deletion of residues in the C-terminal hexapeptide (FSPRLamide) proved that P, R and L played the key parts in initiating the AntpePBANR, the amination to the last C terminal residues which can also likewise impact the activation of AntpePBAN receptor altogether. The mRNA of the AntpePBANR gene demonstrated the most noteworthy transcript levels in pheromone gland followed by fat body. (C) 2018 Elsevier B.V. All rights reserved.