MDA-MB-435 and M14 Cell Lines: Identical but not M14 Melanoma?
MDA-MB-435 and M14 Cell Lines: Identical but not M14 Melanoma?
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DOI：
10.1158/0008-5472.can-09-1528
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发表时间：
2009-07-01
期刊：
影响因子：
11.2
通讯作者：
Chambers, Ann F.
中科院分区：
文献类型：
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作者：
Chambers, Ann F.
在过去的几年里，关于人类MDA-MB-435细胞系的真正起源出现了争议。最初被描述为人类乳腺癌细胞系，随后的表达阵列研究反而表明与黑色素瘤起源一致的基因表达谱。随后的核型和比较基因组杂交研究支持了MDA-MB-435细胞和M14黑色素瘤细胞的当前储备必须是相同的细胞系的想法，并且得出了两种细胞系实际上都是M14黑色素瘤细胞的结论。然而，基于这些数据的另一个结论是，这两种细胞系确实是相同的，但实际上是MDA-MB-435乳腺癌细胞。有证据表明，许多细胞系可以显示“谱系不忠实”，并且基于表达模式不可靠地进行组织类型的分配。本文提供的文献证据与两种细胞系均为M14黑色素瘤来源不一致，但与两种细胞系均为MDA-MB-435乳腺癌来源一致。[Cancer Res 2009;69（13）：5292-3]
A controversy has arisen over the past several years about the true origin of the human MDA-MB-435 cell line. Originally described as a human breast cancer cell line, subsequent expression array studies instead suggested a gene expression profile consistent with a melanoma origin. Subsequent karyotype and comparative genomic hybridization studies supported the idea that current stocks of both MDA-MB-435 cells and M14 melanoma cells must be identical cell lines, and the conclusion was drawn that both cell lines were in fact M14 melanoma cells. However, an alternate conclusion based on these data is that both cell lines are indeed identical, but are in fact MDA-MB-435 breast cancer cells. There is evidence that many cell lines can display "lineage infidelity" and that assignment to tissue type is unreliably made based on expression patterns. Evidence from the literature is presented here that is inconsistent with both lines being of M14 melanoma origin, but rather is consistent with both cell lines being of MDA-MB-435 breast cancer origin. [Cancer Res 2009;69(13):5292-3]