Decline in peripheral blood NKG2D+CD3+CD56+NKT cells in metastatic colorectal cancer patients
Decline in peripheral blood NKG2D+CD3+CD56+NKT cells in metastatic colorectal cancer patients
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DOI：
10.4149/bll_2018_002
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发表时间：
2018-01-01
影响因子：
1.5
通讯作者：
Bayat, B.
中科院分区：
文献类型：
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作者：
Gharagozloo, M.;Rezaei, A.;Bayat, B.
目的：结直肠癌（CRC）是世界范围内癌症死亡的主要原因之一。这种癌症可以分为非转移性和转移性CRC阶段。CD 3 + CD 56 + NKT细胞亚群是外周血中的一个次要T细胞亚群，以直接方式对肿瘤细胞进行杀伤。这些细胞的水平和表面标志物，如NKG 2D在不同的癌症，特别是在CRC.METHODS：我们包括15个非转移性（低级别），11个非转移性（高级别），10个转移性结直肠癌患者和18个健康对照。采用流式细胞仪检测外周血单个核细胞（PBMC）中CD 3 + CD 56 + NKT细胞和NKG 2D + CD 56 + NKT细胞的百分比。我们发现转移性结直肠癌患者外周血中NKG 2D + CD 3 + CD 56+细胞的数量显著低于正常对照组（77.53 +/-5.79%）。vs 90.74 +/-9.84%; p
OBJECTIVE: Colorectal cancer (CRC) is one of the main causes of cancer deaths in the world. This cancer can be divided into non-metastatic and metastatic CRC stages. CD3+CD56+ NKT cell subsets are a minor T cell subset in peripheral blood and conduct the killing of tumor cells in direct manner. Little is obvious about levels and surface markers of these cells such as NKG2D in different cancers, especially in CRC.METHODS: We included 15 non-metastatic (low-grade), 11 non-metastatic (high-grade), 10 metastatic colorectal cancer patients and 18 healthy controls. The percentages of CD3+CD56+ NKT cells and NKG2D+CD56+ NKT cells from samples were analyzed by flow cytometry in peripheral blood mononuclear cells (PBMCs) of samples.RESULTS: We found that there was a significantly lower number of NKG2D+CD3+CD56+ cells in peripheral blood of patients with metastatic colorectal cancer compared with normal controls (77.53 +/- 5.79 % vs 90.74 +/- 9.84 %; p