PROTEOGLYCAN ALTERATIONS FOLLOWING IMMOBILIZATION AND REMOBILIZATION IN THE ARTICULAR-CARTILAGE OF YOUNG CANINE KNEE (STIFLE) JOINT
PROTEOGLYCAN ALTERATIONS FOLLOWING IMMOBILIZATION AND REMOBILIZATION IN THE ARTICULAR-CARTILAGE OF YOUNG CANINE KNEE (STIFLE) JOINT
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DOI：
10.1002/jor.1100080612
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发表时间：
1990-11-01
影响因子：
2.8
通讯作者：
HELMINEN, HJ
中科院分区：
文献类型：
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作者：
SAAMANEN, AM;TAMMI, M;HELMINEN, HJ
在90 °下石膏固定11周后，研究了年轻雌性比格犬膝（后膝关节）软骨上11个部位的蛋白聚糖（PG）分布。屈曲和随后的再活动15周后。制动诱导所有部位PG糖醛酸减少（平均-38%），但最大消耗（-64%）发生在股骨髁的前后端，即，在固定软骨与相对软骨失去接触的位置。在再动员后，糖醛酸的含量仍然低于年龄匹配的对照组（平均-18%），特别是在受固定影响最大的最小接触部位（-33%）。软骨素-6-硫酸盐与软骨素-4-硫酸盐的比率在大多数位置通过固定而降低（平均约14%），并且在再移动后恢复到对照值。在固定化或再活化后，在Sephacryl S-1000凝胶过滤中观察到的聚集PG的百分比没有一致的变化。然而，再动员后，聚集的PG在琼脂糖凝胶电泳中显示出更高比例的较慢的迁移率带，表明更大的单体大小。在对侧负重膝关节中，糖醛酸含量和PG单体类型分布与实验关节中观察到的相同，表明再活动期后达到的状态是由于影响两侧的因素。结果表明，关节面之间的接触力需要保持正常的PG含量和控制机制在每个软骨部位局部工作。限制关节的流动性和负荷在年轻的动物的结论是造成软骨基质的持续变化。此外，在这种研究中使用对侧关节作为唯一对照，尽管实验方便，但似乎并不合适。
The distribution of proteoglycans (PGs) at 11 sites on the knee (stifle joint) cartilage of young female beagle dogs was studied following cast immobilization for 11 weeks in 90.degree. flexion and after a subsequent remobilization for 15 weeks. Immobilization induced a reduction in PG uronic acid at all sites (mean of - 38%), but the greatest depletion (-64%) occurred at the anterior and posterior extremes of the femoral condyles, i.e., at locations where the immobilized cartilage lost contact to the opposing cartilage. Following remobilization, the content of uronic acid remained lower than in the age-matched controls (-18% on average), particularly at the minimum contact sites most affected by immobilization (-33%). The chondroitin-6-sulfate to chondroitin-4-sulfate ratio was reduced by immobilization in most locations (average of -14%) and returned to control values after remobilization. There was no consistent change in the percentage of aggregating PGs observed in Sephacryl S-1000 gel filtration after immobilization or remobilization. However, following remobilization, the aggregating PGs showed an enhanced proportion of the slower mobility band in agarose gel electrophoresis, indicative of a larger monomer size. In the contralateral, load-bearing knee joint, both the uronic acid content and PG monomer type distribution were identical to those observed in the experimental joint, suggesting that the state reached after the remobilization period was due to factor(s) influencing both sides. The results suggest that contact forces between articulating surfaces are required to maintain normal PG content and that the control mechanism works locally at each cartilage site. Restriction of joint mobility and loading in young animals is concluded to cause persistent changes in cartilage matrix. Furthermore, the use of the contralateral joint as the sole control in this kind of studies, although experimentally convenient, seems not to be appropriate.