Monitoring of microenvironmental changes in the major and minor grooves of DNA by dan-modified oligonucleotides
Monitoring of microenvironmental changes in the major and minor grooves of DNA by dan-modified oligonucleotides
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DOI：
10.1021/ol052473m
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发表时间：
2005-12-22
期刊：
影响因子：
5.2
通讯作者：
Majima, T
中科院分区：
文献类型：
--
作者：
Kimura, T;Kawai, K;Majima, T
我们描述了新的环境敏感的荧光探针的合成，以阐明DNA结构。含有荧光载体DAN、(6-(dimethylamino)-2-acylnaphthalene)-modified、DC或DG的寡核苷酸可以通过B-到A-DNA构象转换和RNA杂交来监测DNA主沟和次沟的微环境变化。
We describe the synthesis of new environmentally sensitive fluorescence probes to elucidate DNA structures. DNA oligonucleotides containing fluorophore dan (6-(dimethylamino)-2-acylnaphthalene)-modified dC or dG were able to monitor the microenvironmental changes in both the major and minor grooves of DNA with a B- to A-DNA conformational transition and RNA hybridization.