Transcriptome analysis following enterovirus 71 and coxsackievirus A16 infection in respiratory epithelial cells.
Transcriptome analysis following enterovirus 71 and coxsackievirus A16 infection in respiratory epithelial cells.
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肠道病毒 71 型和柯萨奇病毒 A16 感染呼吸道上皮细胞后的转录组分析
DOI：
10.1007/s00705-020-04821-1
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发表时间：
2020-12
影响因子：
2.7
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Song J;Hu Y;Li W;Li H;Zheng H;Chen Y;Dong S;Liu L
Enterovirus 71 (EV-A71) and coxsackievirus A16 (CV-A16) are the major pathogens responsible for hand, foot and mouth disease (HFMD), but the mechanism by which these viruses cause disease remains unclear. In this study, we used transcriptome sequencing technology to investigate changes in the transcriptome profiles after infection with EV-A71 and CV-A16 in human bronchial epithelial (16HBE) cells. Using systematic bioinformatics analysis, we then searched for useful clues regarding the pathogenesis of HFMD. As a result, a total of 111 common differentially expressed genes were present in both EV-A71- and CV-A16-infected cells. A trend analysis of these 111 genes showed that 91 of them displayed the same trend in EV-A71 and CV-A16 infection, including 49 upregulated genes and 42 downregulated genes. These 91 genes were further used to conduct GO, pathway, and coexpression network analysis. It was discovered that enriched GO terms (such as histone acetylation and positive regulation of phosphorylation) and pathways (such as glycosylphosphatidylinositol (GPI)-anchor biosynthesis and DNA replication) might be closely associated with the pathogenic mechanism of these two viruses, and key genes (such as TBCK and GPC) might be involved in the progression of HFMD. Finally, we randomly selected 10 differentially expressed genes for qRT-PCR to validate the transcriptome sequencing data. The experimental qRT-PCR results were roughly in agreement with the results of transcriptome sequencing. Collectively, our results provide clues to the mechanism of pathogenesis of HFMD induced by EV-A71 and CV-A16.
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影响因子：
8.8
作者：
Enk J;Levi A;Weisblum Y;Yamin R;Charpak-Amikam Y;Wolf DG;Mandelboim O
通讯作者：
Mandelboim O
影响因子：
3.7
作者：
Lee, Jian-Te;Yen, Ting-Yu;Lin, Tzou-Yien
通讯作者：
Lin, Tzou-Yien
影响因子：
1.5
作者：
Amet, Tohti;Lan, Jie;Yu, Qigui
通讯作者：
Yu, Qigui
影响因子：
4
作者：
Capurro, Mariana;Martin, Tonya;Filmus, Jorge
通讯作者：
Filmus, Jorge
miR-1303通过直接靶向MMP9调节BBB通透性并促进CA16感染后CNS损伤
影响因子：
13.2
作者：
Song J;Hu Y;Li H;Huang X;Zheng H;Hu Y;Wang J;Jiang X;Li J;Yang Z;Fan H;Guo L;Shi H;He Z;Yang F;Wang X;Dong S;Li Q;Liu L
通讯作者：
Liu L