The tumor affinity of chlorin e6 and its sonodynamic effects on non-small cell lung cancer
The tumor affinity of chlorin e6 and its sonodynamic effects on non-small cell lung cancer
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二氢卟酚e6的肿瘤亲和力及其对非小细胞肺癌的声动力作用
DOI：
10.1016/j.ultsonch.2012.09.008
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发表时间：
2013-03-01
影响因子：
8.4
通讯作者：
Zhang, Weimin
中科院分区：
文献类型：
--
作者：
Chen, Bei;Zheng, Ruinian;Zhang, Weimin
目的：声动力学疗法（SDT）是一种很有前途的肿瘤治疗新方法。本研究的目的是探讨二氢卟酚e6，光敏剂的肿瘤亲和性，其声动力学效应对NSCLC.Methods：人肺腺癌细胞SPCA-1和荷瘤小鼠SPCA-1异种移植瘤暴露于超声在存在或不存在的二氢卟酚e6。激光扫描共聚焦显微镜观察二氢卟酚e6在细胞内的分布。流式细胞仪分析细胞凋亡和坏死情况。结果：二氢卟酚e6在肿瘤组织中的浓度明显高于瘤旁正常肌肉组织，在18 h时差异最大（肿瘤组织荧光强度是肌肉组织的5.38倍，P < 0.05）。在体内，超声（0.4- 1.6W/cm（2））或二氢卟酚e6（10-40 mg/kg）单独应用对肿瘤生长无明显抑制作用，但超声（1.6W/cm（2））与二氢卟酚e6（SDT）联合应用对肿瘤生长有显著抑制作用（P < 0.05）。腹腔注射二氢卟酚e6 40 mg/kg对血液、肝、肾功能无明显副作用。结论：二氢卟酚e6是一种很有前途的声敏剂，二氢卟酚e6介导的SDT可能为NSCLC的治疗提供新的途径。(C)2012爱思唯尔有限公司版权所有。
Objective: Sonodynamic therapy (SDT) is a promising new approach for cancer therapy. The aim of this study was to investigate the tumor affinity of chlorin e6, a photosensitizer, and its sonodynamic effects on NSCLC.Methods: Human lung adenocarcinoma cells SPCA-1 and mice bearing SPCA-1 tumor xenograft were exposed to ultrasound in the presence or absence of chlorin e6. Chlorin e6 distribution was detected by laser scan confocal microscope. Cell apoptosis and necrosis were studied by flow cytometry analysis. Tumor size and weight were measured after different treatments.Results: The concentration of chlorin e6 in tumor tissue was remarkably higher than that in normal muscle near tumor, and the difference was greatest at 18 h (the fluorescence intensity was 5.38-fold higher in tumor than in muscle, P < 0.05). In vivo, ultrasound (0.4-1.6 W/cm(2)) or chlorin e6 (10-40 mg/kg) alone had no remarkable anti-tumor effects, but the combination of ultrasound (1.6 W/cm(2)) with chlorin e6 (SDT) hampered tumor growth significantly (P < 0.05). Intraperitoneal injection of 40 mg/kg chlorin e6 exerted no notable side effect on blood, liver and kidney function. Flow cytometry analysis showed that chlorin e6-mediated sonodynamic effect was mainly through the induction of cell necrosis.Conclusion: Chlorin e6 is a promising sonosensitizer and chlorin e6-mediated SDT may provide a new approach for NSCLC therapy. (C) 2012 Elsevier B.V. All rights reserved.