Functional conservation of coenzyme Q biosynthetic genes among yeasts, plants, and humans.
Functional conservation of coenzyme Q biosynthetic genes among yeasts, plants, and humans.
复制标题
DOI：
10.1371/journal.pone.0099038
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kawamukai M
中科院分区：
文献类型：
--
作者：
Hayashi K;Ogiyama Y;Yokomi K;Nakagawa T;Kaino T;Kawamukai M
辅酶 Q (CoQ) 是电子传递系统中有氧生长和氧化磷酸化的重要因子。 CoQ的生物合成途径主要是通过对大肠杆菌和酿酒酵母的生化和遗传分析提出的；然而，仅在有限数量的研究中探索了高等真核生物的生物合成途径。我们之前报道了裂殖酵母裂殖酵母中参与 CoQ 合成的几个基因的作用。在这里，我们通过鉴定 CoQ 合成所需的 10 个基因（dps1、dlp1、ppt1 和 coq3-9）来扩展这些发现。生成 CoQ10 缺陷型粟酒裂殖酵母 coq 缺失菌株并对其进行表征。所有突变裂殖酵母菌株都对氧化应激敏感，产生大量硫化物，需要抗氧化剂才能在基本培养基上生长，并且在稳定期无法存活。为了比较裂殖酵母和高等真核生物中 CoQ 的生物合成途径，确定了来自人类和植物（拟南芥）的 CoQ 生物合成基因在功能上补充粟酒裂殖酵母 coq 缺失菌株的能力。除 COQ9 外，所有其他人类和植物 COQ 基因的表达都恢复了裂殖酵母 coq 缺失菌株的 CoQ10 产量，尽管人类 COQ3 和 COQ7 以及拟南芥 COQ6 需要添加线粒体靶向序列。总之，本研究描述了酵母、人类和植物之间 CoQ 生物合成基因的功能保守性。
Coenzyme Q (CoQ) is an essential factor for aerobic growth and oxidative phosphorylation in the electron transport system. The biosynthetic pathway for CoQ has been proposed mainly from biochemical and genetic analyses of Escherichia coli and Saccharomyces cerevisiae; however, the biosynthetic pathway in higher eukaryotes has been explored in only a limited number of studies. We previously reported the roles of several genes involved in CoQ synthesis in the fission yeast Schizosaccharomyces pombe. Here, we expand these findings by identifying ten genes (dps1, dlp1, ppt1, and coq3–9) that are required for CoQ synthesis. CoQ10-deficient S. pombe coq deletion strains were generated and characterized. All mutant fission yeast strains were sensitive to oxidative stress, produced a large amount of sulfide, required an antioxidant to grow on minimal medium, and did not survive at the stationary phase. To compare the biosynthetic pathway of CoQ in fission yeast with that in higher eukaryotes, the ability of CoQ biosynthetic genes from humans and plants (Arabidopsis thaliana) to functionally complement the S. pombe coq deletion strains was determined. With the exception of COQ9, expression of all other human and plant COQ genes recovered CoQ10 production by the fission yeast coq deletion strains, although the addition of a mitochondrial targeting sequence was required for human COQ3 and COQ7, as well as A. thaliana COQ6. In summary, this study describes the functional conservation of CoQ biosynthetic genes between yeasts, humans, and plants.
登录
查看更多内容
DOI：
10.1016/j.bbalip.2008.10.006
发表时间：
2009-01
影响因子：
4.8
作者：
Marbois, Betn;Gin, Peter;Gulmezian, Melissa;Clarke, Catherine F.
通讯作者：
Clarke, Catherine F.
影响因子：
3.8
作者：
Kennedy, Patrick J.;Vashisht, Ajay A.;Russell, Paul
通讯作者：
Russell, Paul
影响因子：
46.9
作者：
Matsuyama, Akihisa;Arai, Ritsuko;Yoshida, Minoru
通讯作者：
Yoshida, Minoru
影响因子：
4.8
作者：
Jonassen, T;Clarke, CF
通讯作者：
Clarke, CF
影响因子：
4.8
作者：
Do, TQ;Hsu, AY;Clarke, CF
通讯作者：
Clarke, CF