Selective Growth of and Electricity Production by Marine Exoelectrogenic Bacteria in Self-Aggregated Hydrogel of Microbially Reduced Graphene Oxide
Selective Growth of and Electricity Production by Marine Exoelectrogenic Bacteria in Self-Aggregated Hydrogel of Microbially Reduced Graphene Oxide
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DOI：
10.3390/c2020015
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发表时间：
2016-05
期刊：
影响因子：
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通讯作者：
N. Yoshida;Y. Goto;Yasushi Miyata
中科院分区：
文献类型：
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作者：
N. Yoshida;Y. Goto;Yasushi Miyata
氧化石墨烯（GO）已被证明可被几种微生物还原。最近的研究表明，GO和GO呼吸细菌（GEOB）在微生物电化学系统中的显着好处，在GO的存在下的生长和电力生产的还原形式的GO（rGO）的电子回收。在这项研究中，我们从沿海样品中富集的BCLB的分布和系统发育多样性的调查在海水环境中的BCLB。X射线光电子能谱（XPS）和四端探测显示，富集的微生物群落（指定为CS培养物）减少GO和自聚集成导电水凝胶复合物与rGO（CS-rGO复合物）。在GO还原过程中，某些细菌种群以依赖于GO呼吸与乙酸盐氧化的方式生长。作为生物标志物的16 S rRNA的高通量测序揭示了脱硫单胞菌属物种占CS培养物中总细菌群体的92%。CS-rGO复合物通过乙酸盐氧化产生电，表现出小于1 Ω/cm 3的电荷转移电阻。因此，这些结果表明脱硫单胞菌属物种可以在rGO上生长并通过还原形式的GO产生电。
Graphene oxide (GO) has been shown to be reduced by several microorganisms. Recent studies of the growth of Geobacter species in the presence of GO and electricity production by recovery of electrons on the reduced form of GO (rGO) have indicated substantial benefits of GO and GO-respiring bacteria (GORB) in microbial electrochemical systems. In this study, we enriched GORB from a coastal sample to investigate the distribution and phylogenetic variety of GORB in seawater environments. X-ray photoelectron spectroscopy (XPS) and four-terminal probing revealed that the enriched microbial community (designated as CS culture) reduced GO and self-aggregated into a conductive hydrogel complex with rGO (the CS-rGO complex). In the process of GO reduction, certain bacterial populations grew in a manner that was dependent on GO respiration coupled with acetate oxidization. High-throughput sequencing of 16S rRNA as a biomarker revealed the predominance of Desulfomonas species at 92% of the total bacterial population in the CS culture. The CS-rGO complex produced electricity with acetate oxidization, exhibiting less than 1 Ω/cm3 of charge transfer resistance. Thus, these results suggested that Desulfomonas species could grow on rGO and produce electricity via the reduced form of GO.