Distribution of estrogen receptor α and β in the mouse central nervous system:: In vivo autoradiographic and immunocytochemical analyses
Distribution of estrogen receptor α and β in the mouse central nervous system:: In vivo autoradiographic and immunocytochemical analyses
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DOI：
10.1002/cne.20128
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发表时间：
2004-05-24
影响因子：
2.5
通讯作者：
Dellovade, TL
中科院分区：
文献类型：
--
作者：
Merchenthaler, I;Lane, MV;Dellovade, TL
虽然雌激素受体β（ER β）免疫反应性在大鼠中枢神经系统中的分布已被报道，但在小鼠中没有这样的数据。本研究使用了体内放射自显影，利用I-125-雌激素，具有相同的结合亲和力的两种受体，以及ER β和ER α的免疫组织化学，调查和比较的两个ER在小鼠中枢神经系统中的分布。使用特异性抗血清。对ER α和ER β的免疫抑制使我们能够评估这些受体对放射自显影检测到的结合的贡献。此外，在卵巢切除的野生型和ER α KO（敲除）小鼠中收集数据，以检查ER α对ER β表达的发育调节。这些研究表明，在小鼠CNS中，结合ER α和ER β的免疫反应性占使用放射自显影观察到结合的所有区域。因此，这些数据有力地表明，雌激素结合在小鼠中枢神经系统中的主要贡献者是ER α和ER β。总之，这些数据为今后的研究提供了解剖学基础，并促进了我们对雌激素在中枢神经系统中作用的理解。此外，由于野生型和ER α KO小鼠的免疫细胞化学图像相似，这些研究表明，ER α的缺乏不会影响ER β在中枢神经系统中的表达。(C)2004 Wiley-Liss，Inc.
Although the distribution of estrogen receptor beta (ERbeta) immunoreactivity in the rat central nervous has been reported, no such data are available in the mouse. The present study used in vivo autoradiography utilizing a I-125-estrogen that has equal binding affinity for both receptors as well as immunohistochemistry for ERbeta and ERalpha, to investigate and compare the distribution of the two ERs in the mouse CNS. The use specific antisera. against ERalpha and ERbeta allowed us to evaluate the contribution of these receptors to the binding detected with autoradiography. In addition, data were collected in ovariectomized wildtype and ERalpha KO (knockout) mice to examine developmental regulation of ERbeta expression by ERalpha. These studies revealed that in the mouse CNS, combining immunoreactivity for ERalpha with that for ERbeta accounted for all regions where binding was seen using autoradiography. Therefore, these data strongly suggest that the major contributors of estrogen binding in the mouse CNS are ERalpha and ERbeta. Together, these data provide an anatomical foundation for future studies and advance our understanding of estrogen action in the CNS. Moreover, since the immunocytochemical images were similar in wildtype and ERalpha KO mice, these studies suggest that the lack of ERalpha does not influence the expression of ERbeta in the central nervous system. (C) 2004 Wiley-Liss, Inc.