Vascular system of intramural leiomyomata revealed by corrosion casting and scanning electron microscopy
Vascular system of intramural leiomyomata revealed by corrosion casting and scanning electron microscopy
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DOI：
10.1093/humrep/deg213
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发表时间：
2003-05-01
影响因子：
6.1
通讯作者：
Miodonski, AJ
中科院分区：
文献类型：
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作者：
Walocha, JA;Litwin, JA;Miodonski, AJ
背景：子宫肌瘤血管系统是女性最常见的良性肿瘤，是控制肿瘤发生、发展的重要因素。然而，还没有用目前最好的腐蚀铸造和扫描电子显微镜相结合的技术对其进行形态研究。方法：取尸检子宫肌瘤标本，用Mercox树脂经传入血管注入固定剂，树脂聚合后腐蚀。用扫描电子显微镜观察所有血管的管型，包括毛细血管。结果：最小肌瘤(1~3 mm)为无血管肌瘤，较大肌瘤(1 Cm)为不规则血管网，密度与正常子宫肌层相近或低于正常肌层。这种肿瘤被一层极其致密的血管层(“血管被膜”)包围着，它是供应和排出肿瘤的较大血管的来源。结论：在子宫肌瘤的发展过程中，原有的血管发生退化，新的血管从肿瘤周围侵入，在肿瘤周围强烈的血管生成，可能是由肿瘤分泌的生长因子促进的，导致形成负责向生长中的肿瘤供应血液的血管被膜。
BACKGROUND: The vascular system of leiomyomata, the most common benign tumours in women, is an important factor controlling development and growth of the tumour. It has not been, however, investigated morphologically using the best currently available technique, corrosion casting combined with scanning electron microscopy. METHODS: Myomatous uteri collected upon autopsy were perfused via afferent vessels with fixative followed by Mercox resin and corroded after polymerization of the resin. The obtained vascular casts visualizing all vessels including capillaries were examined using scanning electron microscopy. RESULTS: The smallest (1-3 mm) fibroids were avascular, in larger ones (1 cm) contained irregular networks of blood vessels with density similar to or lower than that of normal myometrium. Such tumours were surrounded by an extremely dense vascular layer ('vascular capsule') which was the source of larger vessels supplying and draining the tumour. CONCLUSIONS: During development of leiomyoma, the pre-existing blood vessels undergo regression and new vessels invade the tumour from the periphery, where intense angiogenesis, probably promoted by growth factors secreted by the tumour, leads to the formation of a 'vascular capsule' responsible for supply of blood to the growing tumour.