Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis
Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis
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DOI：
10.1002/pld3.219
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发表时间：
2020-05-01
期刊：
影响因子：
3
通讯作者：
Nomura, Takahito
中科院分区：
文献类型：
--
作者：
Yoneyama, Kaori;Akiyama, Kohki;Nomura, Takahito
独角甾内酯（SLs）调节植物生长和逆境反应的重要方面。许多不同类型的SL发生在植物中，但生物合成的完整图景仍不清楚。在拟南芥中，我们已经证明了细胞色素P450单加氧酶MAX1可以将卡拉内酯（CL）转化为卡拉内酯酸（CLA），而2-氧戊二酸依赖的双加氧酶LBO可以将卡拉内酯甲酯（MeCLA）转化为代谢物[MeCLA + 16da]。本研究通过对氘化MeCLA的饲养实验发现，[MeCLA + 16 Da]为羟甲基卡拉丙酮酸酯（1′-HO-MeCLA）。重要的是，这种LBO代谢物在植物中被检测到。有趣的是，其他相关化合物，甲基4-羟基乳酸酯（4-HO-MeCLA）和甲基16-羟基乳酸酯（16-HO-MeCLA），也被发现在lbo突变体中积累。在植物中检测到3-HO-、4-HO-和16-HO-CL，但在max1突变体中没有相应的代谢产物HO-CLAs。这些结果表明，HO-CL衍生物可能是拟南芥中主要的SLs，通过MAX1和LBO产生。
Strigolactones (SLs) regulate important aspects of plant growth and stress responses. Many diverse types of SL occur in plants, but a complete picture of biosynthesis remains unclear. In Arabidopsis thaliana, we have demonstrated that MAX1, a cytochrome P450 monooxygenase, converts carlactone (CL) into carlactonoic acid (CLA) and that LBO, a 2-oxoglutarate-dependent dioxygenase, can convert methyl carlactonoate (MeCLA) into a metabolite called [MeCLA + 16 Da]. In the present study, feeding experiments with deuterated MeCLAs revealed that [MeCLA + 16 Da] is hydroxymethyl carlactonoate (1'-HO-MeCLA). Importantly, this LBO metabolite was detected in plants. Interestingly, other related compounds, methyl 4-hydroxycarlactonoate (4-HO-MeCLA) and methyl 16-hydroxycarlactonoate (16-HO-MeCLA), were also found to accumulate in lbo mutants. 3-HO-, 4-HO-, and 16-HO-CL were detected in plants, but their expected corresponding metabolites, HO-CLAs, were absent in max1 mutants. These results suggest that HO-CL derivatives may be predominant SLs in Arabidopsis, produced through MAX1 and LBO.