The Cohesin Complex Subunit ZmSMC3 Participates in Meiotic Centromere Pairing in Maize
The Cohesin Complex Subunit ZmSMC3 Participates in Meiotic Centromere Pairing in Maize
复制标题
粘连蛋白复合物亚基 ZmSMC3 参与玉米减数分裂着丝粒配对
DOI：
10.1105/tpc.19.00834
复制
发表时间：
--
期刊：
影响因子：
--
通讯作者：
Fangpu Han
中科院分区：
文献类型：
--
作者：
Jing Zhang;Chao Feng;H;ong Su;Yang Liu;Yalin Liu;Fangpu Han
减数分裂由两个高度保守的核分裂组成，这使得真核生物能够通过有性生殖来维持其染色体数目。减数分裂的成功完成依赖于同源染色体配对。减数分裂前期I早期着丝粒的相互作用促进同源染色体配对，但其机制尚不清楚。在这里，我们进行了染色质免疫沉淀-质谱分析的玉米（玉米）花药在早期减数分裂前期I使用抗着丝粒组蛋白H3（CENH 3）的抗体，并确定在此期间的cohesin亚基结构维持染色体3（SMC 3）与CENH 3相互作用。从细线期到粗线期，SMC 3在着丝粒和沿着染色体臂富集，可能促进同源着丝粒之间的相互作用。我们观察到功能失调的SMC 3组装在减数分裂特异性玉米突变体与缺陷的着丝粒配对。在SMC 3 RNA干扰的减数分裂前期Ⅰ，观察到着丝粒配对缺陷，SMC 3与着丝粒弱相关，并且SMC 3不定位于染色体臂。在野生型有丝分裂中，SMC 3与染色质相关，并且从前期到后期在着丝粒处富集。CRISPR-Cas9诱导的Zmsmc 3突变体显示出姐妹染色单体凝聚力的过早丧失和有丝分裂扩散中染色体的错误分离。我们的研究结果表明，除了姐妹染色单体凝聚，ZmSMC 3参与减数分裂着丝粒配对。
Meiosis consists of two highly conserved nuclear divisions, which allow eukaryotes to maintain their chromosome number through sexual reproduction. The successful completion of meiosis depends on homologous chromosome pairing. Centromere interactions during early meiotic prophase I facilitate homologous chromosome pairing, but the underlying mechanism is unclear. Here, we performed chromatin immunoprecipitation-mass spectrometry analysis of maize (Zea mays) anthers during early meiotic prophase I using anti-centromeric histone H3 (CENH3) antibodies and determined that the cohesin subunit Structural Maintenance of Chromosome3 (SMC3) interacts with CENH3 during this period. SMC3 is enriched at centromeres and along chromosome arms in threads from leptotene to pachytene and might promote interactions between homologous centromeres. We observed dysfunctional SMC3 assembly in meiotic-specific maize mutants with defective centromere pairing. InSMC3RNAimeiocytes, centromere pairing defects were observed during early meiotic prophase I, SMC3 was weakly associated with centromeres, and SMC3 did not localize to the chromosome arms. In wild-type mitosis, SMC3 is associated with chromatin and is enriched at centromeres from prophase to anaphase. CRISPR-Cas9-inducedZmsmc3mutants showed premature loss of sister chromatid cohesion and mis-segregation of chromosomes in mitotic spreads. Our findings suggest that in addition to sister chromatid cohesion, ZmSMC3 participates in meiotic centromere pairing.