Identification of the respiratory syncytial virus-induced immunosuppressive factor produced by human peripheral blood mononuclear cells in vitro as interferon-alpha.
Identification of the respiratory syncytial virus-induced immunosuppressive factor produced by human peripheral blood mononuclear cells in vitro as interferon-alpha.
复制标题
人外周血单核细胞体外产生的呼吸道合胞病毒诱导的免疫抑制因子的鉴定为干扰素-α。
DOI：
10.1093/infdis/172.4.919
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
J. H. Pope
中科院分区：
文献类型：
--
作者：
F. Preston;P. L. Beier;J. H. Pope
呼吸道合胞病毒（RSV）在体外可抑制人外周血单核细胞（PBMC）的增殖反应。这种抑制是由细胞外 RSV 诱导因子介导的。在本研究中，该因子被明确确定为干扰素（IFN）-α。 RSV 诱导的 IFN-α 与 PBMC 强烈结合，并且仅在刺激的最初几天内添加时才抑制抗 RSV 增殖反应。然而，白细胞介素 (IL)-2 的产生以及 CD25、CD71 和 HLA-DR 的细胞表面表达均没有随之减少。 RSV 诱导的 IFN-α 的抑制与体外 RSV 诱导的 IL-1、-2 和 -6 或 IFN-γ 的水平无关，也与 IL-1 抑制剂、肿瘤坏死因子-α、前列腺素或 IL-10 的存在无关。 IFN-α 的免疫抑制可能会显着影响 RSV 感染和再感染的结果。
Respiratory syncytial virus (RSV) can inhibit the proliferative response of human peripheral blood mononuclear cells (PBMC) in vitro. This inhibition is mediated by an extracellular RSV-induced factor. In the present study, the factor was clearly identified as interferon (IFN)-alpha. The RSV-induced IFN-alpha bound strongly to PBMC and inhibited the anti-RSV proliferative response only when added within the first few days of stimulation. There was, however, no concomitant decrease in the production of interleukin (IL)-2 nor in the cell surface expression of CD25, CD71, and HLA-DR. Inhibition by RSV-induced IFN-alpha was unrelated to the levels of IL-1, -2, and -6 or of IFN-gamma induced by RSV in vitro or to the presence of IL-1 inhibitor, tumor necrosis factor-alpha, prostaglandin, or IL-10. Immunosuppression by IFN-alpha may significantly affect the outcome of infection and reinfection with RSV.