Acuticoccus yangtzensis gen. nov., sp nov., a Novel Member in the Family Rhodobacteraceae, Isolated from the Surface Water of the Yangtze Estuary
Acuticoccus yangtzensis gen. nov., sp nov., a Novel Member in the Family Rhodobacteraceae, Isolated from the Surface Water of the Yangtze Estuary
复制标题
扬子金针藻属
DOI：
10.1007/s00284-014-0698-1
复制
发表时间：
2015-02-01
影响因子：
2.6
通讯作者：
Xie, Xiabing
中科院分区：
文献类型：
--
作者：
Hou, Lei;Zhang, Yao;Xie, Xiabing
从中国长江口（北纬31°，东经122°）的表层水中分离出一种新细菌，菌株JL1095(T)。细胞为革兰氏阴性、需氧、椭圆形、具有一个峰端并且通过滑动而活动。细胞通过二元裂变分裂。生长发生在 15-50 A 摄氏度（最佳温度为 35 A 摄氏度）、2-10 % (w/v) NaCl（最佳类似于 3 %）和 pH 6.0-9.0（最佳 pH 类似于 7.6）。菌株JL1095(T)能够利用多种单一碳源，如吐温40、吐温80、乙酸、L-阿拉伯糖、d,L-乳酸、尿刊酸、丙酮酸甲酯、α-羟基丁酸、β-羟基丁酸和γ-羟基丁酸。主要细胞脂肪酸为 C-16:0、C-18:0、C-19:0 环欧米茄 8c、C-20:1 欧米茄 7c 和汇总特征 8。整个呼吸泛醌为 Q-10。菌株JL1095(T)基因组DNA G+C含量为51.5%。根据系统发育分析，菌株JL1095(T)在Labrenzia属、Panonibacter属、Stappia属、Wenxnia属和Amaricoccus属占据的进化辐射外围形成单系分支。与关系最密切的菌株Stappia indica B106(T)的序列相似性为92.8%，与模式种Stappia stellulatum IAM 12621(T)的序列相似性为92.6%。根据生化特征、化学分类学和系统发育分析，菌株JL1095(T)被认为是红杆菌科的一个新属。该新菌株被命名为Acuticoccus yangtzensis gen。十一月，SP。十一月扬子金针菌的模式菌株是JL1095(T) (=CGMCC 1.12795 = DSM 28604)。
A novel bacterium, strain JL1095(T), was isolated from the surface water of the Yangtze Estuary, China (31A degrees N, 122A degrees E). Cells were Gram negative, aerobic, oval-shaped with one peak end and motile by gliding. Cells divided by binary fission. Growth occurred at 15-50 A degrees C (optimum at 35 A degrees C), 2-10 % (w/v) NaCl (optimum at similar to 3 %) and pH 6.0-9.0 (optimum at pH similar to 7.6). Strain JL1095(T) was able to utilize various sole-carbon-source, such as Tween 40, Tween 80, acetic acid, l-arabinose, d,l-lactic acid, urocanic acid, methyl-pyruvate, alpha-hydroxy butyric acid, beta-hydroxy butyric acid, and gamma-hydroxy butyric acid. The major cellular fatty acids were C-16:0, C-18:0, C-19:0 omega 8c cyclo, C-20:1 omega 7c, and Summed Feature 8. The whole respiratory ubiquinone was Q-10. The genomic DNA G+C content of strain JL1095(T) was 51.5 %. According to the phylogenetic analysis, strain JL1095(T) formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genera Labrenzia, Pannonibacter, Stappia, Wenxnia, and Amaricoccus. The sequence similarity was 92.8 % with the most closely relating strain Stappia indica B106(T), and 92.6 % with the type species Stappia stellulatum IAM 12621(T). Based on the biochemical characteristics, chemotaxonomy and phylogenetic analysis, strain JL1095(T) is considered to be a novel genus which belongs to the family Rhodobacteraceae. The novel strain is named Acuticoccus yangtzensis gen. nov., sp. nov. The type strain of Acuticoccus yangtzensis is JL1095(T) (=CGMCC 1.12795 = DSM 28604).