Ordered arrays of proteins on graphite observed by scanning tunneling microscopy
Ordered arrays of proteins on graphite observed by scanning tunneling microscopy
复制标题
通过扫描隧道显微镜观察到石墨上有序的蛋白质阵列
DOI：
10.1116/1.585208
复制
发表时间：
1991
影响因子：
1.4
通讯作者：
A. Lenhoff
中科院分区：
文献类型：
--
作者：
L. Haggerty;B. A. Watson;M. Barteau;A. Lenhoff
Two globular proteins, lysozyme and chymotrypsinogen A, were imaged on graphite using a scanning tunneling microscope. In contrast to the isolated molecules typically seen, long‐range order was observed in both of these systems when the protein concentration of the solution deposited on the graphite was sufficient for multilayer coverage. For lysozyme, regular two‐dimensional arrays of protein molecules were seen, with periodicities ranging from about 40 (the approximate size of a lysozyme molecule) to about 150 A. These length scales depend on the lysozyme concentration in the initial solution. For chymotrypsinogen A, two‐dimensional structures that covered a much smaller area of the graphite were observed. The structural features observed suggest such possibilities as protein structure determination using surface structural techniques and epitaxial growth of protein crystals on these two‐dimensional structures.
