Proneural gene-linked neurogenesis in zebrafish cerebellum
Proneural gene-linked neurogenesis in zebrafish cerebellum
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DOI：
10.1016/j.ydbio.2010.03.024
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发表时间：
2010-07-01
影响因子：
2.7
通讯作者：
Hibi, Masahiko
中科院分区：
文献类型：
--
作者：
Kani, Shuichi;Bae, Young-Ki;Hibi, Masahiko
在哺乳动物中，小脑神经元分别归类为谷氨酸能或GABA能，并源自祖细胞，分别表达额定基因ATOH1或PTF1A。在斑马鱼中，三个ATOH1基因，ATOH1A，ATOH1B和ATOH1C在上菱形唇（URL）中以重叠但不同的表达结构域表示：PTF1A在心室区（VZ）中仅表达。使用在ATOH1A和PTF1A调节元件的控制下表达荧光蛋白的转基因线，我们追踪了小脑神经元的谱系。 ATOH1（+）祖细胞不仅会产生颗粒细胞，还引起了前腔菱形脑的神经元。 PTF1A（+）祖细胞产生了Purkinje细胞。谷氨酸胶质剂的olig2（+）e尿牙型细胞主要来自Vz中的Ptf1a（+）祖细胞，但有些来自ATOH1（+）祖细胞（+）的祖细胞，在成年小脑中的url中在VZ中检测到瓣膜小脑和内侧菌群的分子层和ptf1a。胸腔基因表达模式与成年小脑中神经元祖细胞增殖的部位相吻合。我们的数据表明，脊椎动物中的小脑中的胸腔基因相关神经发生在进化上是保守的，并且连续产生的神经元有助于成年斑马鱼小脑中的神经回路。 （c）2010 Elsevier Inc.保留所有权利。
In mammals, cerebellar neurons are categorized as glutamatergic or GABAergic, and are derived from progenitors that express the proneural genes atoh1 or ptf1a, respectively. In zebrafish, three atoh1 genes, atoh1a, atoh1b, and atoh1c, are expressed in overlapping but distinct expression domains in the upper rhombic lip (URL): ptf1a is expressed exclusively in the ventricular zone (VZ). Using transgenic lines expressing fluorescent proteins under the control of the regulatory elements of atoh1a and ptf1a, we traced the lineages of the cerebellar neurons. The atoh1(+) progenitors gave rise not only to granule cells but also to neurons of the anteroventral rhombencephalon. The ptf1a(+) progenitors generated Purkinje cells. The olig2(+) eurydendroid cells, which are glutamatergic, were derived mostly from ptf1a(+) progenitors in the VZ but some originated from the atoh1(+) progenitors in the URL In the adult cerebellum, atoh1a, atoh1b, and atoh1c are expressed in the molecular layer of the valvula cerebelli and of the medial corpus cerebelli, and ptf1a was detected in the VZ. The proneural gene expression patterns coincided with the sites of proliferating neuronal progenitors in the adult cerebellum. Our data indicate that proneural gene-linked neurogenesis is evolutionarily conserved in the cerebellum among vertebrates, and that the continuously generated neurons help remodel neural circuits in the adult zebrafish cerebellum. (C) 2010 Elsevier Inc. All rights reserved.