CHARACTERIZATION OF LEFT-VENTRICULAR COLLAGEN IN THE RAT
CHARACTERIZATION OF LEFT-VENTRICULAR COLLAGEN IN THE RAT
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DOI：
10.1093/cvr/17.1.15
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发表时间：
1983-01-01
影响因子：
10.8
通讯作者：
JACOB, R
中科院分区：
文献类型：
--
作者：
MEDUGORAC, I;JACOB, R
分别用中性盐和稀酸溶液提取正常和肥大大鼠左心室的胶原蛋白，并进行胃蛋白酶消化。稀酸可溶性胶原的产率仅为总胶原的0.3-0.6%；与中性盐的溶解度更低。有限的胃蛋白酶消化允许提取总胶原的50-65%。在3.6 mol cntdot存在的情况下，分析了胃蛋白酶溶解的胶原蛋白中各种类型胶原蛋白分子的分布。L-1尿素在聚丙烯酰胺凝胶电泳的辅助下。在所有未还原和还原的大鼠左心室胶原样品中，胃蛋白酶可溶性胶原的盘状模式显示出现二聚体和三聚体成分，以及高分子量的聚集体。这些观察结果表明存在广泛的链间和分子间交联网络。未还原和还原胃蛋白酶溶解胶原的电泳分析也显示了左心室大鼠胶原的异质性，因为它是I型和III型胶原的混合物。在所有研究的大鼠左心室结缔组织样品中，I型胶原分子组分的比例明显高于III型组分。出生后的生长、衰老和心肌肥大可能影响I型与III型成分的比例。
Collagen of the normal and hypertrophied rat left ventricle was successively extracted with neutral salt and dilute acid solutions and pepsin digestion. The yield of dilute-acid soluble collagen was only 0.3-0.6% of total collagen; the solubility with neutral salt was even lower. Limited pepsin digestion permitted extraction of 50-65% of total collagen. The distribution of the various types of collagen molecules was analyzed in pepsin-solubilized collagen in the presence of 3.6 mol .cntdot. l-1 urea with the aid of electrophoresis on polyacrylamide gels. In all samples of nonreduced and reduced left ventricular collagen of the rat, disc patterns of pepsin-soluble collagen revealed the occurrence of dimeric and trimeric components, as well as aggregates of higher MW. Such observations suggest the presence of an extensive interchain and intermolecular cross-linking network. Electrophoretic analysis of nonreduced and reduced pepsin-solubilized collagen also revealed heterogeneity of left ventricular rat collagen due to its occurrence as a mixture of type I and type III collagen. The proportion of type I collagen molecule components was substantially higher than that of type III components in all investigated samples of rat left ventricular connective tissue. Postnatal growth, aging and myocardial hypertrophy may affect the ratio of type I to type III components.