Effects of dietary cholesterol and phospholipid levels on growth performance, antioxidant capacity, and tissue fatty acid in the juvenile mud crab Scylla paramamosain Estampador, 1950 (Decapoda: Brachyura: Portunidae)
Effects of dietary cholesterol and phospholipid levels on growth performance, antioxidant capacity, and tissue fatty acid in the juvenile mud crab Scylla paramamosain Estampador, 1950 (Decapoda: Brachyura: Portunidae)
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DOI：
10.1093/jcbiol/ruac038
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发表时间：
2022-08
影响因子：
1.1
通讯作者：
Cuiying Chen;Xiaofeng Nie;Yu-Hang Cheng;Jiajian Shen;Pengyuan Zhang;Wenjie Zhong;Shuqi Wang;C. You;Yuanyou Li
中科院分区：
文献类型：
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作者：
Cuiying Chen;Xiaofeng Nie;Yu-Hang Cheng;Jiajian Shen;Pengyuan Zhang;Wenjie Zhong;Shuqi Wang;C. You;Yuanyou Li
为研究饲料胆固醇（CHOL）和磷脂（PL）水平是否存在相互作用，对1950年帕拉莫萨河鼠（Scylla paramamosainestamador, 1950）幼鱼的生长性能、抗氧化和免疫参数产生影响，在聚丙烯池中进行了3 × 3因子饲养试验，试验采用3种饲料水平，分别为干重的0.5%、1.0%、1.5%和0.5%、1.0%、1.5%的CHOL和PL水平。经过8周的饲养试验，0.5% CHOL和1.0或1.5% PL的生长性能最佳，成活率较高。血淋巴CHOL、低密度脂蛋白胆固醇（LDLC）和高密度脂蛋白胆固醇（HDLC）随饲粮CHOL水平的升高而升高，而ldl水平随饲粮PL水平的升高而降低。随着饲粮PL水平的升高，0.5或1.0% CHOL和PL均显著提高了蟹体超氧化物歧化酶（SOD）、谷胱甘肽s -转移酶（GST）、谷胱甘肽过氧化物酶（GSH-Px）活性和总抗氧化能力（T-AOC）。饲粮PL水平升高导致肝胰腺和肌肉中总n-6多不饱和脂肪酸（PUFA）含量升高。对生长性能没有明显的增效作用，但对肝胰脏的血淋巴生化指标和抗氧化能力有增效作用。综上所述，饲粮中0.5% CHOL和1.0 ~ 1.5% PL的适宜水平可维持顺氨粘多糖幼鱼的稳定生长性能和抗氧化能力。
To investigate whether an interaction between the levels of dietary cholesterol (CHOL) and phospholipid (PL) exists in the growth performance, antioxidant, and immune parameters of juvenile Scylla paramamosainEstampador, 1950, a 3 × 3 factorial feeding trial was conducted in polypropylene tanks with nine diets containing three CHOL levels (0.5%, 1.0%, 1.5% of dry weight) and three PL levels (0.5%, 1.0%, 1.5% of dry weight). After the eight-week feeding trial, crabs fed the diets with 0.5% CHOL and 1.0 or 1.5% PL achieved the best growth performance with a relatively higher survival rate. Hemolymph CHOL, low-density lipoprotein cholesterol (LDLC), and high-density lipoprotein cholesterol (HDLC) increased with the increasing dietary CHOL levels, whereas decreased LDLC levels were observed as dietary PL levels increased. With the increasing dietary levels of PL, the activities of superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) were markedly enhanced in crabs fed the basal diets with 0.5 or 1.0% CHOL and PL. Tissue fatty acid composition reflected dietary fatty acids. Increasing dietary PL levels resulted in rising contents of total n-6 polyunsaturated fatty acids (PUFA) in the hepatopancreas and muscle. A synergistic effect between CHOL and PL was not observed on growth performance but such effect was found instead in the biochemical indices of hemolymphs and the antioxidant capacity of the hepatopancreas. These results suggest that proper dietary levels of 0.5% CHOL and 1.0–1.5% PL could maintain solid growth performance and antioxidant capacity of juvenile S. paramamosain.