Yeast RNA polymerase II subunit RPB11 is related to a subunit shared by RNA polymerase I and III.
Yeast RNA polymerase II subunit RPB11 is related to a subunit shared by RNA polymerase I and III.
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发表时间：
1993
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影响因子：
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通讯作者：
N. Woychik;K. McKune;William Arbuthnot Sir Lane;R. Young
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作者：
N. Woychik;K. McKune;William Arbuthnot Sir Lane;R. Young
RNA聚合酶亚基基因的表征揭示了一些亚基是由三种核酶共享的，一些是同源的，而一些是RNA聚合酶I、II或III所特有的。我们在这里报告的酵母RNA聚合酶II亚基RPB 11，这是由一个单拷贝RPB 11基因直接位于上游的拓扑异构酶I基因，TOPI，染色体XV编码的分离和表征。该基因的序列预测了120个氨基酸（13，600道尔顿）的RPB 11亚基，仅比RPB 9多肽短两个氨基酸，在大多数SDS-PAGE条件下与RPB 11共迁移。发现RPB 11是编码与RNA聚合酶I和III共享的必需亚基AC 19密切相关的蛋白质的必需基因。RPB 11含有在其他三种酵母RNA聚合酶亚基和细菌RNA聚合酶亚基α中发现的19个氨基酸片段。一些影响RNA聚合酶组装的突变在该片段内，表明该区域可能在亚基相互作用中发挥作用。由于RPB 11的分离完成了已知的酵母RNA聚合酶II亚基基因的分离，我们简要总结了这12个基因和它们编码的多肽的显著特征。
The characterization of RNA polymerase subunit genes has revealed that some subunits are shared by the three nuclear enzymes, some are homologous, and some are unique to RNA polymerases I, II, or III. We report here the isolation and characterization of the yeast RNA polymerase II subunit RPB11, which is encoded by a single copy RPB11 gene located directly upstream of the topoisomerase I gene, TOPI, on chromosome XV. The sequence of the gene predicts an RPB11 subunit of 120 amino acids (13,600 daltons), only two amino acids shorter than the RPB9 polypeptide, that co-migrates with RPB11 under most SDS-PAGE conditions, RPB11 was found to be an essential gene that encodes a protein closely related to an essential subunit shared by RNA polymerases I and III, AC19. RPB11 contains a 19 amino acid segment found in three other yeast RNA polymerase subunits and the bacterial RNA polymerase subunit alpha. Some mutations that affect RNA polymerase assembly map within this segment, suggesting that this region may play a role in subunit interactions. As the isolation of RPB11 completes the isolation of known yeast RNA polymerase II subunit genes, we briefly summarize the salient features of these twelve genes and the polypeptides that they encode.