The power of correlative microscopy: multi-modal, multi-scale, multi-dimensional.
The power of correlative microscopy: multi-modal, multi-scale, multi-dimensional.
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DOI：
10.1016/j.sbi.2011.06.010
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发表时间：
2011-10
影响因子：
6.8
通讯作者：
Czymmek KJ
中科院分区：
文献类型：
--
作者：
Caplan J;Niethammer M;Taylor RM 2nd;Czymmek KJ
相关显微镜是一种复杂的方法，它结合了通常独立但功能强大的显微镜平台的能力：通常包括但不限于传统的光，共聚焦和超分辨率显微镜，原子力显微镜，透射和扫描电子显微镜，磁共振成像和微/纳米CT（计算机断层扫描）。当针对大群体或组织中的罕见或特定事件时，相关显微镜越来越被认为是首选方法。此外，这种多模式的技术同化提供了互补的、往往是独特的信息，例如来自同一目标样本的内部和外部空间、结构、生物化学和生物物理细节。尖端应用、探针、制备方法、硬件和软件开发的持续发展将使相关显微镜的全部潜力得以实现。
Correlative microscopy is a sophisticated approach that combines the capabilities of typically separate, but powerful microscopy platforms: often including, but not limited, to conventional light, confocal and super-resolution microscopy, atomic force microscopy, transmission and scanning electron microscopy, magnetic resonance imaging and micro/nanoCT (computed tomography). When targeting rare or specific events within large populations or tissues, correlative microscopy is increasingly being recognized as the method of choice. Furthermore, this multi-modal assimilation of technologies provides complementary and often unique information, such as internal and external spatial, structural, biochemical and biophysical details from the same targeted sample. The development of a continuous stream of cutting-edge applications, probes, preparation methodologies, hardware and software developments will enable realization of the full potential of correlative microscopy.