Preparation of multifunctional drug sustained-release system by atomic layer deposition of ZnO in mesoporous titania coating
Preparation of multifunctional drug sustained-release system by atomic layer deposition of ZnO in mesoporous titania coating
复制标题
介孔二氧化钛涂层原子层沉积ZnO制备多功能药物缓释系统
DOI：
10.1016/j.ceramint.2019.12.201
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发表时间：
2020
影响因子：
5.2
通讯作者：
Shen Xinkun
中科院分区：
文献类型：
--
作者：
Wu Xinghai;Yao Litao;Al-Baadani Mohammed A.;Ping Linchao;Wu Shuyi;Al-Bishari Abdullrahman M.;HiiRuYie Kendrick;Deng Zhennan;Liu Jinsong;Shen Xinkun
在以往的研究中，已经证明钛介孔（MP）涂层具有很强的粘结性和优异的抗剥离性，因此是临床植入物的理想选择。此外，MP材料的高孔隙率和比表面积使其具有良好的载药和缓释性能。然而，与未经处理的钛相比，MP样品的生物活性没有明显提高。在本研究中，我们采用原子层沉积（ALD）技术将氧化锌（ZnO）沉积在MP结构上，可以在保持MP结构的同时显著提高MP材料的生物活性。荧光结果显示，ALD循环10次（MP-ALD10）后孔隙度和载药量变化不大，而ALD循环30次（MP-ALD30）后孔隙度和载药量明显下降。细胞结果显示，随着ALD周期的增加（从0到50），相应材料的细胞相容性先升高后降低：MP-ALD10组和MP-ALD30组MC3T3-E1细胞的增殖和分化效果最佳。此外，随着氧化锌的增加，不同MP样品对大肠杆菌和金黄色葡萄球菌的抑菌性能逐渐增强（MP < MP- ald10 < MP- ald30 < MP- ald50）。综上所述，本研究证明10次ZnO沉积可显著增强MP的生物活性，而不影响MP的结构、载药和释药性能，因此MP- ald10比MP更适合于药物装置联合钛植入物的开发。
In previous studies, it has been proven that properties of titanium mesoporous (MP) coatings have a strong bond to substrates and excellent anti-stripping property, thus making it ideal for clinical implants. Also, the high porosity and specific surface area of MP material make it excellent for drug loading and sustained release. However, compared with untreated titanium, the bioactivity of MP samples does not have a significant improvement. In this study, we deposited zinc oxide (ZnO) into/on MP structures using atomic layer deposition (ALD) technology, which would significantly improve the biological activity of MP material while maintaining its structures. The fluorescence results showed that the porosity and drug loading capacity had little change after 10 ALD cycles (MP-ALD10), but decreased significantly after 30 ALD cycles (MP-ALD30). Cell results showed that with the increase of ALD cycle (from 0 to 50), the cytocompatibility of the corresponding materials increased first and then decreased: the optimal proliferation and differentiation of MC3T3-E1 cells were observed in MP-ALD10 and MP-ALD30 groups, respectively. Moreover, it was found that the antibacterial properties of different MP samples againstEscherichia coliand Staphylococcus aureus gradually enhanced with the increase of ZnO (MP < MP-ALD10 < MP-ALD30 < MP-ALD50). Overall, the study proved that 10 cycles of ZnO deposition significantly enhanced the bioactivity of MP without affecting its structure, drug-loading and drug-releasing properties, thus making MP-ALD10 more suitable for the development of drug-device combined titanium implants than MP.