Decreased Cellulase and Xylanase Production in the Fungus Talaromyces cellulolyticus by Disruption of tacA and tctA Genes, Encoding Putative Zinc Finger Transcriptional Factors
Decreased Cellulase and Xylanase Production in the Fungus Talaromyces cellulolyticus by Disruption of tacA and tctA Genes, Encoding Putative Zinc Finger Transcriptional Factors
复制标题
DOI：
10.1007/s12010-015-1497-2
复制
发表时间：
2015-03-01
影响因子：
3
通讯作者：
Ishikawa, Kazuhiko
中科院分区：
文献类型：
--
作者：
Fujii, Tatsuya;Inoue, Hiroyuki;Ishikawa, Kazuhiko
解纤维篮状菌（Talaromyces cellulolyticus，原Acremonium cellulolyticus）是工业生产纤维素酶的重要菌株之一。对T.纤维素酶基因的表达是不充分的，因为仅鉴定了少数与纤维素酶基因表达相关的转录因子。在本研究中，我们破坏了7个假定的转录调节基因，显示与纤维素酶或半纤维素酶调节基因在其他丝状真菌的相似性，并调查这些基因是否与纤维素酶和木聚糖酶的生产。在这7个基因中，有5个（tclA、tbgA、tlaA、tmcA、tclB2）对纤维素酶和木聚糖酶活性的影响较小。另一方面，tacA和tctA分别是纤维素酶的阻遏物ace1和角质酶的诱导物ctf1的同源物，它们的破坏导致了纤维素酶和半纤维素酶产量的降低，这是由于在酶和转录水平上的影响，表明tacA和tctA在T.纤维素分解菌这些结果表明，T. cellulolyticus与其他丝状真菌不同，这意味着T.纤维素分解菌
Talaromyces cellulolyticus (formerly Acremonium cellulolyticus) is one of the important strains for industrial cellulase production. An understanding of the control of cellulase gene expression in T. cellulolyticus is insufficient because only a few transcriptional factors related to cellulase gene expression have been identified. In the present study, we disrupted seven putative transcription regulator genes that showed similarity with cellulase or hemicellulase regulator genes in other filamentous fungi and investigated whether these genes are related to cellulase and xylanase production. Among the seven genes, five (tclA, tbgA, tlaA, tmcA, tclB2) had a smaller effect on cellulase and xylanase activities when culturing with cellulose. On the other hand, disruption of tacA and tctA, which are respectively homologues of ace1 (repressor of cellulase) and ctf1 (inducer of cutinase), led to a decrease in cellulase and hemicellulase production due to effects at both the enzymatic and transcriptional levels, indicating that tacA and tctA have positive roles in cellulase and xylanase production in T. cellulolyticus. These results suggest that cellulase and xylanase gene regulation in T. cellulolyticus differs from that in other filamentous fungi and imply that unknown transcriptional mechanisms function in T. cellulolyticus.