Ca2+-Dependent Protein Kinases and Stress Signal Transduction in Plants
Ca2+-Dependent Protein Kinases and Stress Signal Transduction in Plants
复制标题
DOI：
10.1126/science.274.5294.1900
复制
发表时间：
1996-12
期刊：
影响因子：
56.9
通讯作者：
Jen Sheen
中科院分区：
文献类型：
--
作者：
Jen Sheen
植物的胁迫反应涉及特定基因转录的变化。两个相关的钙依赖性蛋白激酶（CDPK1和CDPK1a）的组成型活性突变体激活应激诱导型启动子，绕过应激信号。其他六种植物蛋白激酶，包括两种不同的CDPKs，未能模拟这种压力信号。这种激活被激酶结构域中的CDPK1突变所消除，并被能够阻断对应激激素脱落酸的反应的组成型活性蛋白磷酸酶2C所减弱。多种功能由不同的CDPK介导。CDPK1和CDPK1a可能是植物逆境信号转导的正调控因子。
Stress responses in plants involve changes in the transcription of specific genes. The constitutively active mutants of two related Ca2+-dependent protein kinases (CDPK1 and CDPK1a) activate a stress-inducible promoter, bypassing stress signals. Six other plant protein kinases, including two distinct CDPKs, fail to mimic this stress signaling. The activation is abolished by a CDPK1 mutation in the kinase domain and diminished by a constitutively active protein phosphatase 2C that is capable of blocking responses to the stress hormone abscisic acid. A variety of functions are mediated by different CDPKs. CDPK1 and CDPK1a may be positive regulators controlling stress signal transduction in plants.