An automated method for measuring lipoprotein lipase and hepatic triglyceride lipase activities in post-heparin plasma.
An automated method for measuring lipoprotein lipase and hepatic triglyceride lipase activities in post-heparin plasma.
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DOI：
10.1016/j.cca.2018.09.022
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发表时间：
2018-12
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通讯作者：
K. Nakajima;T. Machida;S. Imamura;Daisuke Kawase;K. Miyashita;I. Fukamachi;M. Maeda;Yuji Muraba;T. Koga;J. Kobayashi;Takao Kimura;Katsuyuki Nakajima;M. Murakami
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文献类型：
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作者：
K. Nakajima;T. Machida;S. Imamura;Daisuke Kawase;K. Miyashita;I. Fukamachi;M. Maeda;Yuji Muraba;T. Koga;J. Kobayashi;Takao Kimura;Katsuyuki Nakajima;M. Murakami
背景脂蛋白脂酶(LPL)和肝甘油三酯脂酶(HTGL)通过催化甘油三酯的水解，在富含甘油三酯的脂蛋白代谢中起着核心作用。LPL和HTGL活性的定量检测有助于诊断血脂紊乱，但目前还没有一种自动化的方法来检测这些脂肪酶的活性。方法以天然长链脂肪酸2-二甘油酯为底物，采用自动动力学比色法测定肝素后血浆中LPL和HTGL的活性。结果两种方法的稀释曲线范围为0.0~5 0 0 U/L，批内变异系数在5%范围内。在含有潜在干扰物质的样品测试中没有观察到干扰。正常对照组肝素后血浆LPL活性测定范围为30~153 U/L，hTGL活性测定范围为135~431 U/L。结论L磷脂酶和hTGL活性测定方法适用于肝素后血浆LPL和hTGL活性测定。该方法比放射化学法更简便、快速，非常适合于血脂异常的检测。
BackgroundLipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) play a central role in triglyceride-rich lipoprotein metabolism by catalyzing the hydrolysis of triglycerides. Quantification of LPL and HTGL activity is useful for diagnosing lipid disorders, but there has been no automated method for measuring these lipase activities.MethodsThe automated kinetic colorimetric method was used for assaying LPL and HTGL activity in the post-heparin plasma using the natural long-chain fatty acid 2-diglyceride as a substrate. LPL activity was determined with apoCII and HTGL activity was determined without apoCII with 2 channel of auto-analyzer.ResultsThe calibration curve for dilution tests of the LPL and HTGL activity assay ranged from 0.0 to 500 U/L. Within-run CV was obtained within a range of 5%. No interference was observed in the testing of specimens containing potentially interfering substances. The measurement range of LPL activity in the post-heparin plasma was 30–153 U/L, while HTGL activity was 135–431 U/L in normal controls.ConclusionsThe L PL and HTGL activity assays are applicable to quantitating the LPL and HTGL activity in the post-heparin plasma. This assay is more convenient and faster than radiochemical assay and highly suitable for the detection of lipid disorders.