Linkage-linkage disequilibrium dissection of the epigenetic quantitative trait loci (epiQTLs) underlying growth and wood properties in Populus
Linkage-linkage disequilibrium dissection of the epigenetic quantitative trait loci (epiQTLs) underlying growth and wood properties in Populus
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杨树生长和木材特性的表观遗传数量性状位点（epiQTL）的连锁-连锁不平衡剖析
DOI：
10.1111/nph.16220
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发表时间：
2020
期刊：
影响因子：
9.4
通讯作者：
Zhang Deqiang
中科院分区：
文献类型：
--
作者：
Lu Wenjie;Xiao Liang;Quan Mingyang;Wang Qingshi;El Kassaby Yousry A;Du Qingzhang;Zhang Deqiang
越来越多的证据表明，DNA甲基化是可遗传的，并且是导致表型变异的重要标记。利用基于甲基化敏感扩增多态性(MSAP)的分析方法，利用连锁-连锁不平衡作图方法，对一个连锁群体(550个F1后代)和一个自然群体(435个无关个体)的9个生长和木材性质性状的表观遗传结构进行了破译。通过表达数量性状甲基化(EQTM)和核苷酸(EQTN)作图策略，进一步揭示了致病基因中遗传和表观遗传变异之间的相互作用。共有163个表观遗传数量性状基因座(表观QTL；LOD和≥)，解释了1.7-44.5%的表型变异，被定位到包含19个连锁群的高分辨率表观遗传图谱上，这得到了两个群体中显著的MSAP关联(P<；0.001)的支持。与生长调控和木材形成有关的原因基因有23个，其标记位于表观QTL上，与两个群体中相同的性状相关。进一步的eQTN和eQTM作图表明，23个候选基因中的因果遗传变异和表观遗传变异可能相互作用更多的反式基因表达和表型。本研究为研究林木表观遗传结构以及遗传和表观遗传变异之间调控复杂性状的交互作用提供了策略。
Increasing evidence indicates that DNA methylation is heritable and serves as an essential marker contributing to phenotypic variation.Linkage‐linkage disequilibrium mapping was used to decipher the epigenetic architecture underlying nine growth and wood property traits in a linkage population (550 F1progeny) and a natural population (435 unrelated individuals) ofPopulususing methylation‐sensitive amplification polymorphism (MSAP)‐based analysis. The interactions between genetic and epigenetic variants in the causative genes was further unveiled using expression quantitative trait methylation (eQTM) and nucleotide (eQTN) mapping strategies.A total of 163 epigenetic quantitative trait loci (epiQTLs; LOD ≥ 3.0), explaining 1.7–44.5% of phenotypic variations, were mapped to a high‐resolution epigenetic map with 19 linkage groups, which was supported by the significant MSAP associations (P< 0.001) in the two populations. There were 23 causal genes involved in growth regulation and wood formation, whose markers were located in epiQTLs and associated with the same traits in both populations. Further eQTN and eQTM mapping showed that causal genetic and epigenetic variants within the 23 candidate genes may interact more intransin gene expression and phenotype.The present study provides strategies for investigating epigenetic architecture and the interaction between genetic and epigenetic variants modulating complex traits in forest trees.